A sensitive ratiometric fluorescent probe for quantitive detection and imaging of alkaline phosphatase in living cells

被引：40

作者：

Gao, Congcong ^{[1
]}

Zang, Shunping ^{[1
]}

Nie, Longxue ^{[1
]}

Tian, Yong ^{[1
]}

Zhang, Rubo ^{[1
]}

Jing, Jing ^{[1
]}

Zhang, Xiaoling ^{[1
]}

机构：

[1] Beijing Inst Technol, Beijing Key Lab Photoelect Electrophoton Convers, Key Lab Cluster Sci, Sch Chem,Minist Educ, Beijing 100081, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2019年 / 1066卷

基金：

中国国家自然科学基金;

关键词：

Ratiometric; Fluorescent probe; Alkaline phosphatase; SULFUR-DIOXIDE DERIVATIVES; COLORIMETRIC ASSAY; MECHANISM; STRATEGY;

D O I：

10.1016/j.aca.2019.03.043

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a sensitive fluorescence probe Ratio-ALP for in vivo ratiometric imaging of alkaline phosphatase (ALP). The probe was designed by conjugating a naphthalimide with a phosphate group as the responsive moiety for enzymatic reactions. The fluorescence intensity ratio displayed a linear relationship against the concentration of ALP in the concentration range from 0 to 200 U/L, its LOD is as low as 0.25 U/L. Ratio-ALP has good selectivity and sensitivity, high cell permeability, and low cytotoxicity. Furthermore, probe Ratio-ALP was also successfully applied to image endogenous ALP activity in living cells. (C) 2019 Elsevier B.V. All rights reserved.

引用

页码：131 / 135

页数：5

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