Multiplex Real-Time PCR Assay for Detection of Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Suitable in Regions of High MRSA Endemicity

被引：13
作者：
Kim, Jeong-Uk [1 ]
Cha, Choong-Hwan [1 ]
An, Hae-Kyong [1 ]
Lee, Ho-Jun [1 ]
Kim, Mi-Na [2 ]
机构：
[1] Univ Ulsan, Coll Med, Gangneung Asan Hosp, Dept Lab Med, Kangnung, South Korea
[2] Univ Ulsan, Coll Med, Asan Med Ctr, Dept Lab Med, Seoul, South Korea
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2013年 / 51卷 / 03期
关键词：
RAPID DETECTION; CASSETTE; PLATFORM;
D O I：
10.1128/JCM.02495-12
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A multiplex real-time PCR assay that simultaneously detects the mecA, staphylococcal cassette chromosome (SCCmec)-open reading frame X (orfX) junction, and staphylococcal 16S rRNA genes was developed and evaluated using 444 staphylococcal strains. We demonstrated that this assay resulted in fewer false-positive results than a single-locus real-time PCR assay that amplified the SCCmec-orfX junction. This assay would be useful in a clinical laboratory in a region of high endemicity for methicillin-resistant Staphylococcus aureus (MRSA) infections.
引用
收藏
页码：1008 / 1013
页数：6
相关论文
共 30 条
[1]   Does nasal cocolonization by methicillin-resistant coagulase-negative Staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by molecular methods? [J].
Becker, K ;
Pagnier, I ;
Schuhen, B ;
Wenzelburger, F ;
Friedrich, AW ;
Kipp, F ;
Peters, G ;
von Eiff, C .
JOURNAL OF CLINICAL MICROBIOLOGY, 2006, 44 (01) :229-231
[2]   Controlling methicillin-resistant Staphylococcus aureus:: Quantifying the effects of interventions and rapid diagnostic testing [J].
Bootsma, MCJ ;
Diekmann, O ;
Bonten, MJM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (14) :5620-5625
[3]   Multiplex Real-Time PCR for Rapid Staphylococcal Cassette Chromosome mec Typing [J].
Chen, Liang ;
Mediavilla, Jose R. ;
Oliveira, Duarte C. ;
Willey, Barbara M. ;
de Lencastre, Herminia ;
Kreiswirth, Barry N. .
JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (11) :3692-3706
[4]   Hospital-Wide Methicillin-Resistant Staphylococcus aureus Control Program: A 5-Year Follow-up [J].
Chowers, Michal Y. ;
Paitan, Yossi ;
Gottesman, Bat Sheva ;
Gerber, Beatris ;
Ben-Nissan, Yona ;
Shitrit, Pnina .
INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2009, 30 (08) :778-781
[5]   Molecular and epidemiological evidence for spread of multiresistant methicillin-susceptible Staphylococcus aureus strains in hospitals [J].
Donnio, Pierre-Yves ;
Fevrier, Frederic ;
Bifani, Pablo ;
Dehem, Marie ;
Kervegant, Christele ;
Wilhelm, Nathalie ;
Gautier-Lerestif, Anne-Lise ;
Lafforgue, Nathalie ;
Cormier, Michel ;
Le Coustumier, Alain .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2007, 51 (12) :4342-4350
[6]   Partial excision of the chromosomal cassette containing the methicillin resistance determinant results in methicillin-susceptible Staphylococcus aureus [J].
Donnio, PY ;
Oliveira, DC ;
Faria, NA ;
Wilhelm, N ;
Le Coustumier, A ;
de Lencastre, H .
JOURNAL OF CLINICAL MICROBIOLOGY, 2005, 43 (08) :4191-4193
[7]   Nine-year surveillance of methicillin-resistant Staphylococcus aureus in a hospital suggests instability of mecA DNA region in an epidemic strain [J].
Donnio, PY ;
Louvet, L ;
Preney, L ;
Nicolas, D ;
Avril, JL ;
Desbordes, L .
JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (03) :1048-1052
[8]   Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in critical care:: an interventional cohort study [J].
Harbarth, Stephan ;
Masuet-Aumatell, Cristina ;
Schrenzel, Jacques ;
Francois, Patrice ;
Akakpo, Christophe ;
Renzi, Gesuele ;
Pugin, Jerome ;
Ricou, Bara ;
Pittet, Didier .
CRITICAL CARE, 2006, 10 (01)
[9]   Molecular genetics of methicillin-resistant Staphylococcus aureus [J].
Hiramatsu, K ;
Katayama, Y ;
Yuzawa, H ;
Ito, T .
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2002, 292 (02) :67-74
[10]   The emergence and evolution of methicillin-resistant Staphylococcus aureus [J].
Hiramatsu, K ;
Cui, L ;
Kuroda, M ;
Ito, T .
TRENDS IN MICROBIOLOGY, 2001, 9 (10) :486-493
123