Drought induced programmed cell death and associated changes in antioxidants, proteases, and lipid peroxidation in wheat leaves

被引:70
作者
Hameed, A. [1 ]
Goher, M. [1 ]
Iqbal, N. [1 ]
机构
[1] Nucl Inst Agr & Biol, Faisalabad, Pakistan
关键词
MDA; phenolics; proteases; Triticum aestivum; LEAF SENESCENCE; SUPEROXIDE-DISMUTASE; STRESS; GROWTH; ROOTS; L; TOLERANCE; APOPTOSIS; MANNOSE; ANTHERS;
D O I
10.1007/s10535-012-0286-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Two wheat (Triticum aestivum L.) genotypes with varying degree of drought tolerance were used to analyze programmed cell death (PCD) and related biochemical changes under drought stress. Drought induced PCD in leaves, as evident by internucleosomal nDNA fragmentation, was observed in sensitive genotype Nesser. Drought tolerant genotype (FD-83) showed higher peroxidase, superoxide dismutase, and catalase activities and ascorbate content under drought stress compared to sensitive genotype. Total phenolic content increased whereas lipid peroxidation remained un-changed under drought in FD-83. In contrast, drought enhanced the proteases and ascorbate peroxidase activities and lipid peroxidation (MDA content) in Nesser.
引用
收藏
页码:370 / 374
页数:5
相关论文
共 44 条
[1]   Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent [J].
Ainsworth, Elizabeth A. ;
Gillespie, Kelly M. .
NATURE PROTOCOLS, 2007, 2 (04) :875-877
[2]  
Akhter J, 2008, PAK J BOT, V40, P1441
[3]  
Amjad Hameed Amjad Hameed, 2004, International Journal of Agriculture and Biology, V6, P383
[4]  
Amjad Hameed Amjad Hameed, 2005, Caderno de Pesquisa Serie Biologia, V17, P51
[5]   Translocation of cytochrome c from the mitochondria to the cytosol occurs during heat-induced programmed cell death in cucumber plants [J].
Balk, J ;
Leaver, CJ ;
McCabe, PF .
FEBS LETTERS, 1999, 463 (1-2) :151-154
[6]  
BEERS RF, 1952, J BIOL CHEM, V195, P133
[7]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[8]   ASSAY OF CATALASES AND PEROXIDASES [J].
CHANCE, B ;
MAEHLY, AC .
METHODS IN ENZYMOLOGY, 1955, 2 :764-775
[9]   Protein changes during programmed cell death in tobacco [J].
Chaves, I. ;
Alves, M. ;
Carrilho, D. ;
Duque-Magalhaes, M. C. ;
Ricardo, C. P. ;
Regalado, A. P. .
BIOLOGIA PLANTARUM, 2011, 55 (01) :153-158
[10]  
CHEN GX, 1989, PLANT CELL PHYSIOL, V30, P987