Cryo-EM and single particles

被引：29

作者：

Wang, LG ^{[1
]}

Sigworth, FJ ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Cellular & Mol Physiol, New Haven, CT 06510 USA

来源：

PHYSIOLOGY | 2006年 / 21卷

关键词：

D O I：

10.1152/physiol.00045.2005

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Cryoelectronmicroscopy is a method for the imaging of macromolecules in the electron microscope. It was originally developed to determine membrane protein structures from two-dimensional crystals, but more recently "single-particle" techniques have become powerful and popular. Three-dimensional reconstructions are obtained from sets of single-particle images by extensive computer processing; the methods are being applied to many macromolecular assemblies.

引用

页码：13 / 18

页数：6

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