Production of a β-D-glucan-rich extract from Shiitake mushrooms (Lentinula edodes) by an extraction/microfiltration/reverse osmosis (nanofiltration) process

被引：28

作者：

Morales, Diego ^{[1
]}

Smiderle, Fhernanda Ribeiro ^{[2
]}

Jimenez Phis, Adriana ^{[1
]}

Soler-Rivas, Cristina ^{[1
]}

Prodanov, Marin ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Dept Prod & Characterizat Novel Foods, Inst Food Sci Res CIAL CEI CSIC UAM, C Nicolas Cabrera 9,Campus Cantoblanco, E-28049 Madrid, Spain

[2] Univ Fed Parana, Dept Biochem & Mol Biol, Campus Ctr Politecn,CP 19046, BR-81531980 Curitiba, Parana, Brazil

来源：

INNOVATIVE FOOD SCIENCE & EMERGING TECHNOLOGIES | 2019年 / 51卷 / 80-90期

关键词：

beta-D-Glucans; Lentinula edodes; Microfiltration; Nanofiltration; Reverse osmosis; BIOLOGICAL-ACTIVITIES; ENRICHED FRACTIONS; AGARICUS-BISPORUS; EDIBLE MUSHROOMS; POLYSACCHARIDES; PURIFICATION;

D O I：

10.1016/j.ifset.2018.04.003

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A pilot-scale process combining extraction of Shiitake mushroom (Lentinula edodes) powder in water (98 degrees C, 1 h), cross-flow microfiltration and reverse osmosis (nanofiltration) was performed to obtain beta-glucan-rich extracts. Suspensions (45-80 L) obtained after 3 extractions were clarified by microfiltration reducing their turbidity to < 1 NTU. Membrane flux was completely recovered after filtration. One of the clarified extracts was concentrated (to 6-7 L) by reverse osmosis (Nanomax95) and the other two by nanofiltration (Nanomax50 and ALNF99-2517). Different physicochemical parameters (permeate flux, total soluble substances, total suspended particles and electrical conductivity) were monitored during filtration and the bioactive compounds present in the obtained fractions (beta-glucans, total carbohydrates, chitins, eritadenine, lenthionine, ergosterol) were analyzed. The more adequate membrane for Shiitake extract concentration was Nanomax50 because it showed higher filtration flux and higher values of bioactive compounds in the obtained extract than the extracts obtained with the other two membranes. Industrial relevance: This work describes a pilot-scale procedure for obtaining beta-D-glucan-rich extracts from Lentinula edodes (Shiitake mushrooms). The extracts might be used in novel functional foods due to their high content in hypocholesterolemic compounds. The process combines extraction with boiling water, cross-flow membrane clarification and reverse osmosis/nanofiltration concentration of beta-D-glucans. The procedure is scalable to industrial level.

引用

页码：80 / 90

页数：11