Effects of Atmospheric-pressure Non-thermal Plasma Jets on Enzyme Solutions

被引：27

作者：

Attri, Pankaj ^{[1
]}

Venkatesu, Pannuru ^{[1
]}

Kaushik, Nagendra ^{[2
,3
]}

Han, Yong Gyu ^{[2
,3
]}

Nam, Chul Joo ^{[2
,3
]}

Choi, Eun Ha ^{[2
,3
]}

Kim, Key Sun ^{[4
]}

机构：

[1] Univ Delhi, Dept Chem, Delhi 110007, India

[2] Kwangwoon Univ, Plasma BioSci Res Ctr, Seoul 139701, South Korea

[3] Kwangwoon Univ, Dept Electrophys, Seoul 139701, South Korea

[4] Korean Inst Sci & Technol, Ctr Neurosci, Seoul 136791, South Korea

来源：

JOURNAL OF THE KOREAN PHYSICAL SOCIETY | 2012年 / 60卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

Enzyme; Cold atmospheric-pressure plasma jets; Circular dichroism and fluorescence; ALPHA-CHYMOTRYPSIN; SERINE PROTEASES; IONIC LIQUID; UREA; STERILIZATION; MECHANISM;

D O I：

10.3938/jkps.60.959

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

In order to study the wide scope of structural information of biomolecules in the presence of biocompatible cold atmospheric pressure plasma jets (CAPJ), we used circular dichroism (CD) and fluorescence experiments. Biomolecules are very sensitive and are highly complex systems, exhibiting a substantial degree of structural variability in their folded state. In the present investigation, we compared the compatiblity of alpha-chymotrypsin (CT) in the presence of biocompatible CAPJ. Using circular dichroism (CD) and fluorescence experiments, we describe the associated structural changes in CT after a 5 min treatment with CAPJ. The modifications in the secondary structure of this beta/beta protein were quantified by using the CD spectra. A reasonable decrease was observed for beta-strands after treatment with CAPJ as compared to buffer, which contributes to its deterioration power. The implications of our results from studies delineating the effects of CAPJ on enzyme solutions are discussed.

引用

页码：959 / 964

页数：6

共 27 条

[1] A protic ionic liquid attenuates the deleterious actions of urea on α-chymotrypsin [J].