Interaction and diffusion of gold nanoparticles in bovine serum albumin solutions

被引：27

作者：

Kohli, Indermeet ^{[1
]}

Alam, Sharmine ^{[1
]}

Patel, Bhavdeep ^{[1
]}

Mukhopadhyay, Ashis ^{[1
]}

机构：

[1] Wayne State Univ, Dept Phys & Astron, Detroit, MI 48201 USA

来源：

APPLIED PHYSICS LETTERS | 2013年 / 102卷 / 20期

关键词：

POLYMER-SOLUTIONS; PROTEIN; FLUORESCENCE; LUMINESCENCE; NANORODS; BEHAVIOR; BINDING; CANCER; CORONA; SIZE;

D O I：

10.1063/1.4807672

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We studied the interactions of small sized, radius of 2.5-10 nm, gold nanoparticles (AuNPs) with bovine serum albumin (BSA). Fluctuation correlation spectroscopy (FCS) was used to monitor the changes in the Brownian diffusion of these NPs. Our results indicate a BSA monolayer formation at the NP surface with a thickness of 3.8 nm. The thickness of the adsorbed layer was independent of NP size. Best fit was obtained by the anticooperative binding model with the Hill coefficient of n = 0.63. Dissociation constant (K-D) increases with particle size indicating stronger interaction of BSA with smaller sized NPs. (C) 2013 AIP Publishing LLC.

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页数：4

相关论文

共 25 条

[1] Nanoparticle interaction with plasma proteins as it relates to particle biodistribution, biocompatibility and therapeutic efficacy [J].

Aggarwal, Parag ;

Hall, Jennifer B. ;

McLeland, Christopher B. ;

Dobrovolskaia, Marina A. ;

McNeil, Scott E. .

ADVANCED DRUG DELIVERY REVIEWS, 2009, 61 (06) :428-437

[2] Probing BSA binding to citrate-coated gold nanoparticles and surfaces [J].

Brewer, SH ;

Glomm, WR ;

Johnson, MC ;

Knag, MK ;

Franzen, S .

LANGMUIR, 2005, 21 (20) :9303-9307

[3] The glass transition behavior of the globular protein bovine serum albumin [J].

Brownsey, GJ ;

Noel, TR ;

Parker, R ;

Ring, SG .

BIOPHYSICAL JOURNAL, 2003, 85 (06) :3943-3950

[4] Mobility of Nonsticky Nanoparticles in Polymer Liquids [J].

Cai, Li-Heng ;

Panyukov, Sergey ;

Rubinstein, Michael .

MACROMOLECULES, 2011, 44 (19) :7853-7863

[5]

Casals E, 2012, NANOMEDICINE-UK, V7, P1917, DOI [10.2217/NNM.12.169, 10.2217/nnm.12.169]

[6] Time Evolution of the Nanoparticle Protein Corona [J].

Casals, Eudald ;

Pfaller, Tobias ;

Duschl, Albert ;

Oostingh, Gertie Janneke ;

Puntes, Victor .

ACS NANO, 2010, 4 (07) :3623-3632

[7] Contrasting Effect of Gold Nanoparticles and Nanorods with Different Surface Modifications on the Structure and Activity of Bovine Serum Albumin [J].

Chakraborty, Soumyananda ;

Joshi, Prachi ;

Shanker, Virendra ;

Ansari, Z. A. ;

Singh, Surinder P. ;

Chakrabarti, Pinak .

LANGMUIR, 2011, 27 (12) :7722-7731

[8] Interaction of colloidal gold nanoparticles with human blood: effects on particle size and analysis of plasma protein binding profiles [J].

Dobrovolskaia, Marina A. ;

Patri, Anil K. ;

Zheng, Jiwen ;

Clogston, Jeffrey D. ;

Ayub, Nader ;

Aggarwal, Parag ;

Neun, Barry W. ;

Hall, Jennifer B. ;

McNeil, Scott E. .

NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2009, 5 (02) :106-117

[9] In Situ Measurement of Bovine Serum Albumin Interaction with Gold Nanospheres [J].

Dominguez-Medina, Sergio ;

McDonough, Steven ;

Swanglap, Pattanawit ;

Landes, Christy F. ;

Link, Stephan .

LANGMUIR, 2012, 28 (24) :9131-9139

[10] Two-photon luminescence imaging of cancer cells using molecularly targeted gold nanorods [J].

Durr, Nicholas J. ;

Larson, Timothy ;

Smith, Danielle K. ;

Korgel, Brian A. ;

Sokolov, Konstantin ;

Ben-Yakar, Adela .

NANO LETTERS, 2007, 7 (04) :941-945