Optimized Design and Synthesis of a Cell-Permeable Biarsenical Cyanine Probe for Imaging Tagged Cytosolic Bacterial Proteins

被引：20

作者：

Fu, Na ^{[1
]}

Xiong, Yijia ^{[1
]}

Squier, Thomas C. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Div Biol Sci, Fundamental Sci Directorate, Richland, WA 99352 USA

来源：

BIOCONJUGATE CHEMISTRY | 2013年 / 24卷 / 02期

关键词：

FUSION PROTEINS; RNA-POLYMERASE; ESCHERICHIA-COLI; AFFINITY PROBE; COVALENT; IDENTIFICATION; MOLECULES; FLUOROPHORES; LOCALIZATION; CALMODULIN;

D O I：

10.1021/bc300619m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To optimize cellular delivery and specific labeling of tagged cytosolic proteins by biarsenical fluorescent probes built around a cyanine dye (Cy3) scaffold, we have systematically varied the polarity of the N-alkyl chain (i.e., 4-5 methylene groups appended by a sulfonate or methoxy ester moiety) and arsenic capping reagent (ethanedithiol versus benzenedithiol). Optimal live-cell labeling and visualization of tagged cytosolic proteins is reported using an ethanedithiol capping reagent with the uncharged methoxy ester functionalized N-alkyl chains. These measurements demonstrate the general utility of this new class of photostable and highly fluorescent biarsenical probes based on the cyanine dye scaffold for in vivo labeling of tagged cellular proteins for live cell imaging measurements of protein dynamics.

引用

页码：251 / 259

页数：9

共 49 条

[21] Selective Recognition of Protein Tetraserine Motifs with a Cell-Permeable, Pro-fluorescent Bis-boronic Acid [J].

Halo, Tiffany L. ;

Appelbaum, Jacob ;

Hobert, Elissa M. ;

Balkin, Daniel M. ;

Schepartz, Alanna .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2009, 131 (02) :438-+

[22] Coupling the distribution of RNA polymerase to global gene regulation and the dynamic structure of the bacterial nucleoid in Escherichia coli [J].

Jin, Ding Jun ;

Cabrera, Julio E. .

JOURNAL OF STRUCTURAL BIOLOGY, 2006, 156 (02) :284-291

[23] Protamine-induced permeabilization of cell envelopes of gram-positive and gram-negative bacteria [J].

Johansen, C ;

Verheul, A ;

Gram, L ;

Gill, T ;

Abee, T .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (03) :1155-1159

[24] A fusion of disciplines: Chemical approaches to exploit fusion proteins for functional genomics [J].

Johnsson, N ;

Johnsson, K .

CHEMBIOCHEM, 2003, 4 (09) :803-810

[25] Directed evolution of O6-alkylguanine-DNA alkyltransferase for efficient labeling of fusion proteins with small molecules in vivo [J].

Juillerat, A ;

Gronemeyer, T ;

Keppler, A ;

Gendreizig, S ;

Pick, H ;

Vogel, H ;

Johnsson, K .

CHEMISTRY & BIOLOGY, 2003, 10 (04) :313-317

[26] Confocal Fluorescence Anisotropy and FRAP Imaging of α-Synuclein Amyloid Aggregates in Living Cells [J].

Julia Roberti, M. ;

Jovin, Thomas M. ;

Jares-Erijman, Elizabeth .

PLOS ONE, 2011, 6 (08)

[27] Practical syntheses of dyes for difference gel electrophoresis [J].

Jung, ME ;

Kim, WJ .

BIOORGANIC & MEDICINAL CHEMISTRY, 2006, 14 (01) :92-97

[28] An esterase from Escherichia coli with a sequence similarity to hormone-sensitive lipase [J].

Kanaya, S ;

Koyanagi, T ;

Kanaya, E .

BIOCHEMICAL JOURNAL, 1998, 332 :75-80

[29] Labeling of fusion proteins with synthetic fluorophores in live cells [J].

Keppler, A ;

Pick, H ;

Arrivoli, C ;

Vogel, H ;

Johnsson, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (27) :9955-9959

[30] A general method for the covalent labeling of fusion proteins with small molecules in vivo [J].

Keppler, A ;

Gendreizig, S ;

Gronemeyer, T ;

Pick, H ;

Vogel, H ;

Johnsson, K .

NATURE BIOTECHNOLOGY, 2003, 21 (01) :86-89

← 1 2 3 4 5 →