Optimized Design and Synthesis of a Cell-Permeable Biarsenical Cyanine Probe for Imaging Tagged Cytosolic Bacterial Proteins

被引：20

作者：

Fu, Na ^{[1
]}

Xiong, Yijia ^{[1
]}

Squier, Thomas C. ^{[1
]}

机构：

[1] Pacific NW Natl Lab, Div Biol Sci, Fundamental Sci Directorate, Richland, WA 99352 USA

来源：

BIOCONJUGATE CHEMISTRY | 2013年 / 24卷 / 02期

关键词：

FUSION PROTEINS; RNA-POLYMERASE; ESCHERICHIA-COLI; AFFINITY PROBE; COVALENT; IDENTIFICATION; MOLECULES; FLUOROPHORES; LOCALIZATION; CALMODULIN;

D O I：

10.1021/bc300619m

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To optimize cellular delivery and specific labeling of tagged cytosolic proteins by biarsenical fluorescent probes built around a cyanine dye (Cy3) scaffold, we have systematically varied the polarity of the N-alkyl chain (i.e., 4-5 methylene groups appended by a sulfonate or methoxy ester moiety) and arsenic capping reagent (ethanedithiol versus benzenedithiol). Optimal live-cell labeling and visualization of tagged cytosolic proteins is reported using an ethanedithiol capping reagent with the uncharged methoxy ester functionalized N-alkyl chains. These measurements demonstrate the general utility of this new class of photostable and highly fluorescent biarsenical probes based on the cyanine dye scaffold for in vivo labeling of tagged cellular proteins for live cell imaging measurements of protein dynamics.

引用

页码：251 / 259

页数：9

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