Quartromicin Biosynthesis: Two Alternative Polyketide Chains Produced by One Polyketide Synthase Assembly Line

被引:38
作者
He, Hai-Yan [1 ]
Pan, Hai-Xue [1 ]
Wu, Long-Fei [1 ]
Zhang, Bei-Bei [1 ]
Chai, Han-Bo [1 ]
Liu, Wen [1 ]
Tang, Gong-Li [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Organ Chem, State Key Lab Bioorgan & Nat Prod Chem, Shanghai 200032, Peoples R China
来源
CHEMISTRY & BIOLOGY | 2012年 / 19卷 / 10期
基金
中国国家自然科学基金;
关键词
DIELS-ALDER REACTION; GENE-CLUSTER; MACROPHOMATE SYNTHASE; ANTIVIRAL ANTIBIOTICS; CARRIER PROTEIN; DOMAINS; FERMENTATION; SPECIFICITY; INSIGHTS; REVEALS;
D O I
10.1016/j.chembiol.2012.07.024
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The antiviral compounds quartromicins represent unique members of a family of spirotetronate natural products. In this study, a biosynthetic gene cluster of quartromicins was identified by degenerate primer PCR amplification of specific genes involved in the biosynthesis of the tetronate moiety. The biochemical results confirmed that 1,3-bisphosphoglycerate was incorporated into the tetronate ring, and the intermediates of this ring were also reconstructed in vitro. The data also suggested a module skipping strategy for the production of two alternative polyketide chains by the same polyketide synthase assembly line. These findings set the stage for further investigations of the stereodivergent intermolecular cyclization mechanism, and highlight how nature has constructed this type of C2 symmetric molecule through intermolecular dimerization.
引用
收藏
页码:1313 / 1323
页数:11
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