Enhanced yield of ethylene glycol production from D-xylose by pathway optimization in Escherichia coli

被引：35

作者：

Cabulong, Rhudith B. ^{[1
]}

Valdehuesa, Kris Nino G. ^{[1
]}

Ramos, Kristine Rose M. ^{[1
]}

Nisola, Grace M. ^{[1
]}

Lee, Won-Keun ^{[2
]}

Lee, Chang Ro ^{[2
]}

Chung, Wook-Jin ^{[1
]}

机构：

[1] Myongji Univ, E2FTC, DEST, Myongji Ro 116, Yongin 17058, Gyeonggi Do, South Korea

[2] Myongji Univ, Div Biosci & Bioinformat, Myongji Ro 116, Yongin 17058, Gyeonggi Do, South Korea

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2017年 / 97卷

基金：

新加坡国家研究基金会;

关键词：

Aldehyde reductase; Ethylene glycol; Dahms pathway; D-xylose; Metabolic engineering; YjgB; MICROBIAL-PRODUCTION; REDUCTASE; ACID; 1,2,4-BUTANETRIOL; BIOSYNTHESIS; METABOLISM; EXPRESSION; CONVERSION; ETHANOL; SYSTEMS;

D O I：

10.1016/j.enzmictec.2016.10.020

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The microbial production of renewable ethylene glycol (EG) has been gaining attention recently due to its growing importance in chemical and polymer industries. EG has been successfully produced biosynthetically from D-xylose through several novel pathways. The first report on EG biosynthesis employed the Dahms pathway in Escherichia coli wherein 71% of the theoretical yield was achieved. This report further improved the EG yield by implementing metabolic engineering strategies. First, D-xylonic acid accumulation was reduced by employing a weak promoter which provided a tighter control over Xdh expression. Second, EG yield was further improved by expressing the YjgB, which was identified as the most suitable aldehyde reductase endogenous to E. coli. Finally, cellular growth, D-xylose consumption, and EG yield were further increased by blocking a competing reaction. The final strain (WTXB) was able to reach up to 98% of the theoretical yield (25% higher as compared to the first study), the highest reported value for EG production from D-xylose. (C) 2016 Elsevier Inc. All rights reserved.

引用

页码：11 / 20

页数：10

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