Effects of Myeloablation, Peripheral Chimerism, and Whole-Body Irradiation on the Entry of Bone Marrow-Derived Cells Into the Brain

被引：57
作者：
Lampron, Antoine
Lessard, Martine
Rivest, Serge [1 ,2 ]
机构：
[1] Univ Laval, Fac Med, Lab Endocrinol & Genom, CHUL Res Ctr, Quebec City, PQ G1K 7P4, Canada
[2] Univ Laval, Fac Med, Dept Mol Med, Quebec City, PQ G1K 7P4, Canada
来源：
CELL TRANSPLANTATION | 2012年 / 21卷 / 06期
关键词：
Microglia; Central nervous sysem (CNS); Neuroimmunology; Hematopoietic stem cell; Irradiation; Chemotherapy; Bone marrow-derived cells; Innate immunity; CENTRAL-NERVOUS-SYSTEM; BARRIER PERMEABILITY; ALZHEIMERS-DISEASE; STEM-CELLS; MICROGLIA; EXPRESSION; CLONING; PLAY;
D O I：
10.3727/096368911X593154
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Understanding how bone marrow-derived cells (BMDCs) enter the central nervous system (CNS) is critical for the development of therapies for brain-related disorders using hematopoietic stem cells. We investigated the brain damages and blood-brain barrier (BBB) modification following either whole-body irradiation or a myeloablative chemotherapy regimen in mice, and the capacity for these treatments to induce the entry of BMDCs into the CNS. Neither treatment had a lasting effect on brain integrity and both were equally efficient at achieving myeloablation. Injection of bone marrow cells from green fluorescent protein (GFP) transgenic mice was able to completely repopulate the hematopoietic niche in the circulation and in hematopoietic organs (thymus and spleen). However, GFP(+) cells only entered the brain following whole-body irradiation. We conclude that myeloablation, damages to the brain integrity, or the BBB and peripheral chimerism are not responsible for the entry of BMDCs into the CNS following irradiation.
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收藏
页码：1149 / 1159
页数：11
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