Mapping of disease-related genes in Japanese pear using a molecular linkage map with RAPD markers

被引:53
作者
Iketani, H [1 ]
Abe, K [1 ]
Yamamoto, T [1 ]
Kotobuki, K [1 ]
Sato, Y [1 ]
Saito, T [1 ]
Terai, O [1 ]
Matsuta, N [1 ]
Hayashi, T [1 ]
机构
[1] Natl Inst Fruit Tree Sci, Tsukuba, Ibaraki 3058605, Japan
关键词
linkage map; Japanese pear; RAPD; pear scab; black spot; disease-related gene;
D O I
10.1270/jsbbs.51.179
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Molecular linkage maps of disease-related genes in the Japanese pear cultivars 'Kinchaku' and 'Kosui' with RAPD markers were constructed. Using the pseudo-testcross method, two separate maps were constructed from the segregation data of 82 F-1 individuals. The linkage map for 'Kinchaku' consisted of 120 loci in 18 linkage groups spanning 768 cM, while that for 'Kosui' contained 78 loci in 22 linkage groups extending over 508 cM. The resistance allele of pear scab and the susceptibility allele of black spot were mapped in different linkage groups in 'Kinchaku'. Compared with the reports on apple genome mapping, we estimated that these maps covered at least about a half of the total pear genome.
引用
收藏
页码:179 / 184
页数:6
相关论文
共 44 条
[41]  
WILLIAMS EB, 1966, P AM SOC HORTIC SCI, V88, P52
[42]   Screening apples for OPD20/600 using sequence-specific primers [J].
Yang, H ;
Korban, SS .
THEORETICAL AND APPLIED GENETICS, 1996, 92 (02) :263-266
[43]   A randomly amplified polymorphic DNA (RAPD) marker tightly linked to the scab-resistance gene V-f in apple [J].
Yang, HY ;
Korban, SS ;
Kruger, J ;
Schmidt, H .
JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE, 1997, 122 (01) :47-52
[44]   IDENTIFICATION OF A RAPD MARKER LINKED TO THE VF GENE FOR SCAB RESISTANCE IN APPLES [J].
YANG, HY ;
KRUGER, J .
EUPHYTICA, 1994, 77 (1-2) :83-87