Pinopodes, leukemia inhibitory factor, integrin-β3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss

被引：61

作者：

Xu, Bufang ^{[1
]}

Sun, Xiaoxi ^{[2
]}

Li, Lu ^{[2
]}

Wu, Ling ^{[1
]}

Zhang, Aijun ^{[1
]}

Feng, Yun ^{[1
]}

机构：

[1] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Reprod Med Ctr, Shanghai 200025, Peoples R China

[2] Hosp Fudan Univ, Shanghai JiAi, Genet & IVF Inst, Shanghai, Peoples R China

来源：

FERTILITY AND STERILITY | 2012年 / 98卷 / 02期

基金：

上海市自然科学基金;

关键词：

Endometrial receptivity; implantation; pinopodes; recurrent pregnancy loss; IMPLANTATION WINDOW; EMBRYO IMPLANTATION; UTERINE RECEPTIVITY; INFERTILE WOMEN; MISCARRIAGE; MUC1; PHASE; EPITHELIUM; LIF; GLYCOFORMS;

D O I：

10.1016/j.fertnstert.2012.04.032

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To compare pinopode morphology and coverage, and expression of leukemia inhibitory factor (LIF), integrin-beta 3, and mucin-1 (MUC1) in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss (RPL) versus normal fertile women. Design: Exploratory clinical study. Setting: University-affiliated assisted reproductive center. Patient(s): Women undergoing assisted reproduction at centers of reproductive medicine in two university teaching hospitals. Intervention(s): Endometrial biopsy samples were obtained from women with RPL (test group, n = 30) and normal fertile women (control group, n = 26) 7 days after the day of luteinizing hormone peak. Pinopode morphology and coverage were assessed by use of scanning electron microscopy. Expression of LIF, integrin beta 3, and MUC1 were evaluated by immunohistochemical staining. Main Outcome Measure(s): Pinopode morphology and coverage, and the expression of LIF, integrin-beta 3, and MUC1 in both groups. Result(s): Pinopode morphology and coverage, and the expression of LIF and integrin-beta 3 showed no statistically significant differences between the two groups. The expression of MUC1 was statistically significantly reduced in the glandular epithelial cells and endometrial lumen of the RPL group compared with controls. Conclusion(s): The morphology and the coverage of pinopodes and expression of LIF and integrin-beta 3 did not change, but MUC1 expression was statistically significantly reduced in women with RPL compared with normal women. (Fertil Steril (R) 2012;98:389-95. (C) 2012 by American Society for Reproductive Medicine.)

引用

页码：389 / 395

页数：7

共 36 条

[1] Human uterodomes (pinopods) do not display pinocytotic function [J].

Adams, SM ;

Gayer, N ;

Hosie, MJ ;

Murphy, CR .

HUMAN REPRODUCTION, 2002, 17 (08) :1980-1986

[2] Coexpression of pinopodes and leukemia inhibitory factor, as well as its receptor, in human endometrium [J].

Aghajanova, L ;

Stavreus-Evers, A ;

Nikas, Y ;

Hovatta, O ;

Landgren, BM .

FERTILITY AND STERILITY, 2003, 79 :808-814

[3]

Aplin JD, 1996, AM J REPROD IMMUNOL, V35, P261

[4] Human endometrial MUC1 carries keratan sulfate: characteristic glycoforms in the luminal epithelium at receptivity [J].

Aplin, JD ;

Hey, NA ;

Graham, RA .

GLYCOBIOLOGY, 1998, 8 (03) :269-276

[5] Reproductive failure due to spontaneous abortion and recurrent miscarriage [J].

Bulletti, C ;

Flamigni, C ;

Giacomucci, E .

HUMAN REPRODUCTION UPDATE, 1996, 2 (02) :118-136

[6] Embryo implantation [J].

Carson, DD ;

Bagchi, I ;

Dey, SK ;

Enders, AC ;

Fazleabas, AT ;

Lessey, BA ;

Yoshinaga, K .

DEVELOPMENTAL BIOLOGY, 2000, 223 (02) :217-237

[7] αvβ3 integrin expression and pinopod formation in normal and out-of-phase endometria of fertile and infertile women [J].

Creus, M ;

Ordi, J ;

Fábregues, F ;

Casamitjana, R ;

Ferrer, B ;

Coll, E ;

Vanrell, JA ;

Balasch, J .

HUMAN REPRODUCTION, 2002, 17 (09) :2279-2286

[8] Leukemia inhibitory factor (LIF) and LIF receptor expression in human endometrium suggests a potential autocrine paracrine function in regulating embryo implantation [J].

Cullinan, EB ;

Abbondanzo, SJ ;

Anderson, PS ;

Pollard, JW ;

Lessey, BA ;

Stewart, CL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (07) :3115-3120

[9]

DeSouza MM, 1999, J REPROD IMMUNOL, V45, P127, DOI 10.1016/S0165-0378(99)00046-7

[10] MUC16 is lost from the uterodome (pinopode) surface of the receptive human endometrium: In vitro evidence that MUC16 is a barrier to trophoblast adherence [J].

Gipson, Ilene K. ;

Blalock, Timothy ;

Tisdale, Ann ;

Spurr-Michaud, Sandra ;

Allcorn, Sara ;

Stavreus-Evers, Anneli ;

Gemzell, Kristina .

BIOLOGY OF REPRODUCTION, 2008, 78 (01) :134-142

← 1 2 3 4 →