Effect of trapidil in myocardial ischemia-reperfusion injury in rabbit

被引：13
作者：
Liu, MingJie [1 ,2 ]
Sun, Qi [1 ,3 ]
Wang, Qiang [3 ]
Wang, Xiuying [4 ]
Lin, Peng [4 ]
Yang, Ming [4 ]
Yan, Yuanyuan [4 ]
机构：
[1] Jilin Univ, Hosp 1, Changchun 130000, Jilin, Peoples R China
[2] Inner Mongolia Univ Nationalities, Tongliao 028000, Inner Mongolia, Peoples R China
[3] 202 Mil Hosp China, Shenyang, Peoples R China
[4] Liaoning Prov Inst Food & Drug Control, Shenyang 110000, Liaoning, Peoples R China
来源：
INDIAN JOURNAL OF PHARMACOLOGY | 2014年 / 46卷 / 02期
关键词：
Apoptosis index; ischemia-reperfusion; myocardial ischemia-reperfusion injury; trapidil; CELL APOPTOSIS; INFARCTION; PROTEIN; MODEL; PROTECTION;
D O I：
10.4103/0253-7613.129320
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Objectives: To evaluate the cardioprotective effects of trapidil on myocardial ischemia-reperfusion injury (MIRI) in rabbits. Materials and Methods: Rabbits were subjected to 40 min of myocardial ischemia followed by 120 min of reperfusion. Blood for superoxide dismutase (SOD) and malondialdehyde (MDA) were estimated. At the end of reperfusion, the rabbits were sacrificed and the hearts were isolated for histological examination. An apoptotic index (AI) was determined using the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick-end-labeling (TUNEL) method. The expression of apoptosis-related proteins Bax and Bcl-2 was analyzed using immunohistochemistry. Statistical analyses were performed by one-way analysis of variance (ANOVA), P < 0.05 considered statistically significant Results: Trapidil caused a significant (P < 0.05) increase in SOD activity, as decreased MDA levels and significantly (P < 0.05) reduced the expression of Bax as compared with the ischemia-reperfusion (IR) control group. Conclusion: Trapidil may attenuate the myocardial damage produced by IR injury and offer potential cardioprotective action.
引用
收藏
页码：207 / 210
页数：4
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