Isolation, characterization and cross-amplification of polymorphic microsatellite loci in the threatened endemic Mammillaria crucigera (Cactaceae)

被引:14
作者
Solorzano, Sofia [1 ]
Cortes-Palomec, Aurea C. [2 ]
Ibarra, Arturo [1 ]
Davila, Patricia [1 ]
Oyama, Ken [2 ]
机构
[1] Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biol Tecnol & Prototipos, Tlalnepantla 54090, Estado Mexico, Mexico
[2] Univ Nacl Autonoma Mexico, CIEco, Antigua Carretera Patzcuaro, Morelia 58190, Michoacan, Mexico
关键词
Cactaceae; Mammillaria crucigera; microsatellite; CACTUS;
D O I
10.1111/j.1755-0998.2008.02422.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cactus Mammillaria crucigera is a threatened species endemic to central Mexico. As a means of assessing population genetic status of these species, eight microsatellite markers were developed. These primers were tested in 40 individual from two wild populations. The results showed that these primers will be useful to describe population structure and aid to the conservation of species. The eight primers were tested in other Mammillaria species and most of them showed successful amplification.
引用
收藏
页码:156 / 158
页数:3
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