Experimental Transmission of African Swine Fever (ASF) Low Virulent Isolate NH/P68 by Surviving Pigs

被引:97
作者
Gallardo, C. [1 ]
Soler, A. [1 ]
Nieto, R. [1 ]
Sanchez, M. A. [1 ]
Martins, C. [2 ]
Pelayo, V. [1 ]
Carrascosa, A. [3 ]
Revilla, Y. [3 ]
Simon, A. [1 ]
Briones, V. [1 ]
Sanchez-Vizcaino, J. M. [4 ]
Arias, M. [1 ]
机构
[1] INIA CISA, Ctr Invest Sanidad Anim, European Union Reference Lab African Swine Fever, Madrid, Spain
[2] Univ Tecn Lisbon FMV UTL, Fac Med Vet, Lisbon, Portugal
[3] CSIC UAM, Agencia Estatal Consejo Super Invest Cient, Ctr Biol Mol Severo Ochoa, Madrid, Spain
[4] Univ Complutense Madrid, Dept Sanidad Anim, VISAVET, Fac Vet, Madrid, Spain
关键词
African swine fever; carrier state; virus transmission; DOMESTIC PIGS; CHRONIC PNEUMONIA; VIRUS ISOLATE; WILD BOAR; PCR ASSAY; PATHOGENESIS; RESISTANCE; EXCRETION; PATHOLOGY;
D O I
10.1111/tbed.12431
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
African swine fever (ASF) has persisted in Eastern Europe since 2007, and two endemic zones have been identified in the central and southern parts of the Russian Federation. Moderate- to low-virulent ASF virus isolates are known to circulate in endemic ASF-affected regions. To improve our knowledge of virus transmission in animals recovered from ASF virus infection, an experimental invivo study was carried out. Four domestic pigs were inoculated with the NH/P68 ASF virus, previously characterized to develop a chronic form of ASF. Two additional in-contact pigs were introduced at 72days post-inoculation (dpi) in the same box for virus exposure. The inoculated pigs developed a mild form of the disease, and the virus was isolated from tissues in the inoculated pigs up to 99dpi (pigs were euthanized at 36, 65, 99 and 134dpi). In-contact pigs showed mild or no clinical signs, but did become seropositive, and a transient viraemia was detected at 28days post-exposure (dpe), thereby confirming late virus transmission from the inoculated pigs. Virus transmission to in-contact pigs occurred at four weeks post-exposure, over three months after the primary infection. These results highlight the potential role of survivor pigs in disease maintenance and dissemination in areas where moderate- to low-virulent viruses may be circulating undetected. This study will help design better and more effective control programmes to fight against this disease.
引用
收藏
页码:612 / 622
页数:11
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