Multicenter evaluation of proposed standardized procedure for antifungal susceptibility testing of filamentous fungi

被引：200

作者：

EspinelIngroff, A

Bartlett, M

Bowden, R

Chin, NX

Cooper, C

Fothergill, A

McGinnis, MR

Menezes, P

Messer, SA

Nelson, PW

Odds, FC

Pasarell, L

Peter, J

Pfaller, MA

Rex, JH

Rinaldi, MG

Shankland, GS

Walsh, TJ

Weitzman, I

机构：

[1] INDIANA UNIV,SCH MED,INDIANAPOLIS,IN 46202

[2] FRED HUTCHINSON CANC RES CTR,SEATTLE,WA 98104

[3] COLUMBIA PRESBYTERIAN MED CTR,NEW YORK,NY 10032

[4] UNIV TEXAS,MED BRANCH,GALVESTON,TX 77555

[5] UNIV TEXAS,HLTH SCI CTR,AUDIE L MURPHY MEM VET HOSP,LAB SERV,SAN ANTONIO,TX 78284

[6] UNIV IOWA,COLL MED,DEPT PATHOL,IOWA CITY,IA 52242

[7] UNIV TEXAS,SCH MED,CTR STUDY EMERGING & REEMERGING PATHOGENS,DEPT INTERNAL MED,DIV INFECT DIS,HOUSTON,TX 77030

[8] JANSSEN RES FDN,B-2340 BEERSE,BELGIUM

[9] NCI,MYCOL UNIT,BETHESDA,MD 20892

[10] NCI,IMMUNOCOMPROMISED HOST SECT,PEDIAT BRANCH,BETHESDA,MD 20892

[11] REG MYCOL REFERENCE LAB,GLASGOW,LANARK,SCOTLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1997年 / 35卷 / 01期

关键词：

D O I：

10.1128/JCM.35.1.139-143.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multicenter study was conducted to expand the generation and analysis of data that supports the proposal of a reference method for the antifungal susceptibility testing of filamentous fungi. Broth microdilution MICs of amphotericin B and itraconazole were determined in 11 centers against 30 coded duplicate pairs of Aspergillus spp., Fusarium spp., Pseudallescheria boydii, and Rhizopus arrhizus. The effect of inoculum density (approximately 10(3) and 10(4) CFU/ml), incubation time (24, 48, and 72 h), and procedure of MIC determination (conventional and colorimetric [Alamar Blue] evaluation of growth inhibition) on intra- and interlaboratory agreement was analyzed. Based on intra- (97 to 100%) and interlaboratory (94 to 95%) agreement for both drugs, the overall optimal testing conditions identified were determination of colorimetric MICs after 48 to 72 h of incubation with an inoculum density of approximately 10(4) CFU/ml. These testing conditions are proposed as guidelines for a reference broth microdilution method.

引用

页码：139 / 143

页数：5

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