Ex vivo and in vitro assessment of anti-inflammatory activity of seed β-conglutin proteins from Lupinus angustifolius

被引：17

作者：

Lima-Cabello, Elena ^{[1
]}

Morales-Santana, Sonia ^{[2
,3
]}

Foley, Rhonda C. ^{[4
]}

Melser, Su ^{[5
]}

Alche, Victor ^{[6
]}

Siddique, Kadambot H. M. ^{[7
,8
]}

Singh, Karam B. ^{[4
,7
,8
]}

Alche, Juan D. ^{[1
]}

Jimenez-Lopez, Jose C. ^{[1
,7
,8
]}

机构：

[1] CSIC, Estn Expt Zaidin, Dept Biochem Cell & Mol Biol Plants, Prof Albareda 1, E-18008 Granada, Spain

[2] Univ Hosp San Cecilio, Biomed Res Inst Granada IBS Granada, CIBER Fragil & Hlth Aging CIBERFFS, Endocrinol Unit,Endocrinol Div, Dr Oloriz 16, E-18012 Granada, Spain

[3] Univ Hosp San Cecilia, Biomed Res Inst Granada IBS Granada, Proteom Res Serv, Dr Oloriz 16, E-18012 Granada, Spain

[4] CSIRO, CELS, Agr & Food, 147 Underwood Ave, Wembly, WA 6014, Australia

[5] Univ Bordeaux, INSERM, NeuroCtr Magendie, Team Endocannabinoids & Neuroadaptat,U1215, 146 Rue Leo Saignat, F-33076 Bordeaux, France

[6] Hlth Ctr Villanueva De Las Torres, Andalusian Hlth Syst, E-18539 Granada, Spain

[7] Univ Western Australia, UWA Inst Agr, 35 Stirling Highway, Nedlands, WA 6009, Australia

[8] Univ Western Australia, Sch Agr & Environm, 35 Stirling Highway, Nedlands, WA 6009, Australia

来源：

JOURNAL OF FUNCTIONAL FOODS | 2018年 / 40卷

关键词：

Anti-inflammatory proteins; beta-conglutins; chemokine CCL5; IL-1; beta; iNOS; Lupinus angustifolius; Vicilin; ISLET INFLAMMATION; NITRIC-OXIDE; CYTOKINES; ANTIOXIDANT; ACTIVATION; TYPE-1; ALPHA;

D O I：

10.1016/j.jff.2017.11.040

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

The development of functional food ingredients using legume seed proteins has potential for nutraceutical use. We purified recombinant beta-conglutin proteins (r beta 1 to r beta 4, and r beta 6) from narrow-leafed lupin using affinity-chromatography, and evaluated their anti-inflammatory activity using ex vivo and in vitro systems. r beta 1, r beta 3, and r beta 6 produced lower levels of pro-inflammatory mediators such as nitric oxide (about -34.0-fold in all cases), iNOS mRNA (-7.15, -7.97, -7.41-fold), interleukin 1 beta (-12.05, -11.64, -12.16-fold), chemokine CCL5 (-16.0, -18.0, -19.0-fold), and cytokines including TNF-alpha, INF-gamma, IL-1 beta, IL-2, IL-6, IL-8, and IL-12 (beta 1: -28, -100, -8, -2, -49, -45, -127-fold; beta 3: -22, -400, -9, -3, -33, -10, -2.54-fold; beta 6:-72, -122, -11, -3, -2000, -13, -338-fold). These results suggest that the beta 1, beta 3, and beta 6 conglutins have potential as functional food components in nutraceuticals and that can provide alternative therapies for the prevention and treatment of inflammatory related diseases.

引用

页码：510 / 519

页数：10

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