Use of the SOS-chromotest spot assay as a screening system for detecting genotoxic compounds in crude plant extracts

被引：0
作者：
Baez, DA
Vallejo, GZ
Sánchez, PC
Valle, MB
Chilpa, RR
Estrada, MJ
机构：
[1] Univ Nacl Autonoma Mexico, Inst Quim, Mexico City 04510, DF, Mexico
[2] Escuela Nacl Ciencias Biol, Dept Quim Organ, IPN, Mexico City 11340, DF, Mexico
[3] Inst Nacl Invest Nucl, Dept Biol, Mexico City, DF, Mexico
来源：
ATLA-ALTERNATIVES TO LABORATORY ANIMALS | 2002年 / 30卷 / 01期
关键词：
SOS-chromotest; genotoxic compounds; Senna; flavonoids; antitumoral compounds;
D O I：
暂无
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
An SOS-chromotest spot assay was used to detect genotoxic compounds in crude plant extracts. The method allows simultaneous testing of extracts from different species in either a liquid or a solid crystalline form. Extracts from two species of the genus Senna, native to the state of Morelos, Mexico, were assayed. Four genotoxic compounds were isolated, and were identified as quercetin and rutin from S. wislizeni, and 5,7-di-O-methylrutin and 5,7-di-O-methylquercetin from S. skinneri. The SOS-chromotest spot assay proved to be useful for activity-guided fractionation at the beginning of screening for genotoxic compounds in crude plant extracts.
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页码：87 / 92
页数：6
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