Butelase-Mediated Ligation as an Efficient Bioconjugation Method for the Synthesis of Peptide Dendrimers

被引：36

作者：

Cao, Yuan ^{[1
]}

Nguyen, Giang K. T. ^{[1
]}

Chuah, Samuel ^{[1
]}

Tam, James P. ^{[1
]}

Liu, Chuan-Fa ^{[1
]}

机构：

[1] Nanyang Technol Univ, Sch Biol Sci, 60 Nanyang Dr, Singapore 637551, Singapore

来源：

BIOCONJUGATE CHEMISTRY | 2016年 / 27卷 / 11期

基金：

新加坡国家研究基金会;

关键词：

ANTIMICROBIAL PEPTIDES; PROTEINS; VACCINE; DESIGN; ORIENTATION; CONJUGATION; CHEMISTRY; LIGASE;

D O I：

10.1021/acs.bioconjchem.6b00538

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Herein we report a novel enzymatic bioconjugation method to prepare peptide dendrimers. Under the catalysis of a newly discovered peptide ligase, butelase 1, peptide dendrimers of di-, tetra-, and octabranches were successfully synthesized using thiodepsipeptides as acyl donors for ligation with lysyl dendrimeric scaffolds. The efficient assembly of the highly clustered dendrimeric structure highlighted the versatility of butelase 1. We also showed that our synthetic antibacterial peptide dendrimers containing an RLYR motif are highly potent and broadly active against antibiotic-resistant strains.

引用

页码：2592 / 2596

页数：5

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