Enzyme Based Amperometric Biosensor for Adenine Determination

被引：6

作者：

Boka, Beata ^{[1
]}

Adanyi, Nora ^{[2
]}

Virag, Diana ^{[1
]}

Frebort, Ivo ^{[3
]}

Kiss, Attila ^{[1
]}

机构：

[1] Eszterhazy Karoly Coll, EGERFOOD Reg Knowledge Ctr, H-3300 Eger, Hungary

[2] Cent Environm & Food Sci Res Inst, H-1022 Budapest, Hungary

[3] Palacky Univ, Ctr Reg Hana Biotechnol & Agr Res, CZ-78371 Olomouc, Czech Republic

来源：

ELECTROANALYSIS | 2013年 / 25卷 / 01期

关键词：

Biosensors; Electrochemistry; Enzymes; Adenine; Adenine deaminase; CAPILLARY-ZONE-ELECTROPHORESIS; PERFORMANCE LIQUID-CHROMATOGRAPHY; GUANINE; ELECTRODE; PURINE; NUCLEOSIDES; OXIDATION; ADENOSINE; ASSAY; BEER;

D O I：

10.1002/elan.201200387

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzyme-based amperometric biosensor was developed for the rapid determination of adenine in biological and food samples. Recombinant adenine deaminase (EC 3.5.4.2) from Schizosaccharomyces pombe was co-immobilized on graphite electrode with xanthine oxidase (EC 1.17.3.2), peroxidase (EC 1.11.1.7) and an Os mediator. Optimal working conditions were pH 7.0 and -150 mV vs. Ag/AgCl. The novel method was characterized by a detection limit of 1 mu M and a linear measuring range of 0.0050.1 mM. Different beer and tea samples were analyzed by the newly elaborated adenine biosensor. The results obtained correlate with the adenine content measured by the reference HPLC method.

引用

页码：237 / 242

页数：6

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