Polymorphisms inBMPR-IBgene and their association with litter size trait in Chinese Hu sheep

被引：23

作者：

Yang, Zhenwei ^{[1
,2
]}

Yang, Xinyue ^{[1
,2
]}

Liu, Guangbin ^{[1
,2
]}

Deng, Ming ^{[1
,2
]}

Sun, Baoli ^{[1
,2
]}

Guo, Yongqing ^{[1
,2
]}

Liu, Dewu ^{[1
,2
]}

Li, Yaokun ^{[1
,2
]}

机构：

[1] South China Agr Univ, Coll Anim Sci, Guangzhou, Peoples R China

[2] South China Agr Univ, Natl Local Joint Engn Res Ctr Livestock & Poultry, Guangzhou, Peoples R China

来源：

ANIMAL BIOTECHNOLOGY | 2022年 / 33卷 / 02期

关键词：

Hu sheep; BMPR-IB; SNPs; litter size trait; association analysis; BMPR-IB GENE; OVULATION RATE; FECB GENE; INDIGENOUS SHEEP; BOOROOLA FECB; MUTATION; FECUNDITY; GROWTH; BREEDS; EXPRESSION;

D O I：

10.1080/10495398.2020.1789158

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Identification and utilization of sheep major fecundity genes offer opportunities for the increase in litter size, as well as the improvement of production efficiency in livestock industry.BMPR-IBgene belongs to theTGF-beta superfamily, and is also considered as a regulator for sheep reproductive performance due to its involvement in the mammalian gametogenesis pathway. This study aimed to detect the variations ofBMPR-IBgene in Hu sheep (N = 934) and to evaluate their effects on the litter size trait. qRT-PCR results showed that the mRNA expression level ofBMPR-IBin kidney was the highest. And in the tissues of ovary and pituitary, the expression levels of prolific group were significantly higher than that of non-prolific group (p < 0.05). Through DNA sequencing and PCR-RFLP, three SNPs were identified in the genomic region ofBMPR-IBgene; the individuals with CC in g.29362047T > C, AA in g.29427689G > A and GG in FecB had better fecundity characterization. Additionally, association analysis indicated that two diplotypes of Hap2/2 and Hap2/4 showed larger litter size. Overall, our results verified several useful markers which would contribute to further development of sheep breeding strategies.

引用

页码：250 / 259

页数：10

共 37 条

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