Cellulase production and oil palm empty fruit bunch saccharification by a new isolate of Trichoderma koningii D-64

被引：21
作者：
Wang, Zunsheng [1 ]
Ong, Hui Xin [1 ]
Geng, Anli [1 ]
机构：
[1] Ngee Ann Polytech, Sch Life Sci & Chem Technol, Singapore 599489, Singapore
来源：
PROCESS BIOCHEMISTRY | 2012年 / 47卷 / 11期
关键词：
Trichoderma koningii D-64; Trichoderma reesei Rut C30; Cellulolytic enzymes; beta-Glucosidase; Oil palm empty fruit bunch; Hydrolysis; ENZYMATIC-HYDROLYSIS; ENHANCED PRODUCTION; BETA-GLUCOSIDASE; BIOCONVERSION; PURIFICATION; CELLULOSICS; INDUCTION; ETHANOL; LACTOSE; MUTANT;
D O I：
10.1016/j.procbio.2012.07.001
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new strain Trichoderma koningii D-64 was isolated from Singapore soil samples. It produced cellulase, xylanase, and laccase on a variety of carbon sources. Enzyme activities of 3.8 +/- 0.3, 40.3 +/- 5.1, 6.6 +/- 0.3 and 98.8 +/- 10.3 U/mL were respectively obtained for FPase, CMCase, beta-glucosidase and xylanase in a mixture of 1% cellulose and 2% wheat bran. About 70-95% saccharification efficiency of oil palm empty fruit bunch was obtained using T. koningii D-64 enzymes alone without the supplement of any other commercial enzymes. Strain T. koningii D-64 is therefore a potential cellulase producer for the efficient lignocellulosic biomass conversion to fermentable sugars. (C) 2012 Elsevier Ltd. All rights reserved.
引用
收藏
页码：1564 / 1571
页数：8
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