Installing Logic Gates in Permeability Controllable Polyelectrolyte-Carbon Nitride Films for Detecting Proteases and Nucleases

被引：43

作者：

Chen, Lichan ^{[1
,2
,3
]}

Zeng, Xiaoting ^{[1
,2
]}

Dandapat, Anirban ^{[3
]}

Chi, Yuwu ^{[1
,2
,4
]}

Kim, Donghwan ^{[3
,4
]}

机构：

[1] Fuzhou Univ, MOE Key Lab Anal & Detect Technol Food Safety, State Key Lab Photocatalysis Energy & Environm, Fuzhou 350108, Fujian, Peoples R China

[2] Fuzhou Univ, Coll Chem, Fuzhou 350108, Fujian, Peoples R China

[3] Nanyang Technol Univ, Sch Chem & Biomed Engn, Singapore 637457, Singapore

[4] Sungkyunkwan Univ, Sch Chem Engn, Seoul 16419, South Korea

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 17期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

HYDROGEN-EVOLUTION; ENERGY-TRANSFER; DNA; TRANSPORT; ION; DEGRADATION; ACTIVATION; NANOSHEETS; CAPSULES; POLYMER;

D O I：

10.1021/acs.analchem.5b01916

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Proteases and nucleases are enzymes heavily involved in many important biological processes, such as cancer initiation, progression, and metastasis; hence, they are indicative of potential diagnostic biomarkers. Here, we demonstrate a new label free and sensitive electrochemiluminescent (ECL) sensing strategy for protease and nuclease assays that utilize target-triggered desorption of programmable polyelectrolyte films assembled on graphite-like carbon nitride (g-C3N4) film to regulate the diffusion flux of a coreactant. Furthermore, we have built Boolean logic gates OR and AND into the polyelectrolyte films, capable of simultaneously sensing proteases and nucleases in a complicated system by breaking it into simple functions. The developed intelligent permeability controlled enzyme sensor may prove valuable in future medical diagnostics.

引用

页码：8851 / 8857

页数：7

共 43 条

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