The interactome of a family of potential methyltransferases in HeLa cells

被引:49
作者
Ignatova, Valentina V. [1 ]
Jansen, Pascal W. T. C. [2 ]
Baltissen, Marijke P. [2 ]
Vermeulen, Michiel [2 ]
Schneider, Robert [1 ]
机构
[1] Helmholtz Zentrum Munchen, Deutsch Forschungszentrum Gesundheit & Umwelt Gmb, Inst Funct Epigenet, Ingolstaedter Landstr 1, D-85764 Neuherberg, Germany
[2] Radboud Univ Nijmegen, Oncode Inst, Radboud Inst Mol Life Sci, Dept Mol Biol,Fac Sci, Geert Grooteplein 30, NL-6525 GA Nijmegen, Netherlands
关键词
MESSENGER-RNA; NUCLEAR-RNA; PROTEIN; DNA; COMPLEX; IDENTIFICATION; PURIFICATION; TMEM126A; TARGETS; NURD;
D O I
10.1038/s41598-019-43010-2
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Human methytransferase like proteins (METTL) are part of a large protein family characterized by the presence of binding domains for S-adenosyl methionine, a co-substrate for methylation reactions. Despite the fact that members of this protein family were shown or predicted to be DNA, RNA or protein methyltransferases, most METTL proteins are still poorly characterized. Identification of complexes in which these potential enzymes act could help to understand their function(s) and substrate specificities. Here we systematically studied interacting partners of METTL protein family members in HeLa cells using label-free quantitative mass spectrometry. We found that, surprisingly, many of the METTL proteins appear to function outside of stable complexes whereas others including METTL7B, METTL8 and METTL9 have high-confidence interaction partners. Our study is the first systematic and comprehensive overview of the interactome of METTL protein family that can provide a crucial resource for further studies of these potential novel methyltransferases.
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页数:9
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