Correlation of fractalkine concentrations in serum and synovial fluid with the radiographic severity of knee osteoarthritis

被引：18
作者：
Zou, Yucong [1 ]
Li, Yikai [1 ]
Lu, Lu [1 ]
Lin, You [1 ]
Liang, Weiming [1 ]
Su, Zhiyong [2 ]
Wang, Xusheng [2 ]
Yang, Hui [2 ]
Wang, Jianming [3 ]
Yu, Chengfu [4 ]
Huo, Liwei [5 ]
Ye, Yongliang [6 ]
机构：
[1] Southern Med Univ, Dept Tradit Chinese Med, Guangzhou 510515, Guangdong, Peoples R China
[2] Zhuhai Peoples Hosp, Dept Traumat Orthoped, Zhuhai, Peoples R China
[3] Zhuhai Peoples Hosp, Dept Radiol, Zhuhai, Peoples R China
[4] Southern SinoWest Hosp, Dept Radiol, Guangzhou, Guangdong, Peoples R China
[5] Guangzhou Orthoped Hosp, Dept Emergency, Guangzhou, Guangdong, Peoples R China
[6] Guangzhou Orthoped Hosp, Dept Orthoped, Guangzhou, Guangdong, Peoples R China
来源：
ANNALS OF CLINICAL BIOCHEMISTRY | 2013年 / 50卷 / 06期
关键词：
Fractalkine; serum; synovial fluid; severity; osteoarthritis; RHEUMATOID-ARTHRITIS; CD16(+) MONOCYTES; FIBROBLASTS; CHEMOKINE;
D O I：
10.1177/0004563213480494
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background Fractalkine has been detected in synovial fluid (SF) from osteoarthritis (OA) patients. This study aims to examine the relation of fractalkine concentrations in serum and SF with the radiographic severity of OA. Methods Fractalkine concentrations of serum and SF were measured using an enzyme-linked immunosorbent assay method in 223 patients with knee OA and 165 healthy controls. The progression of OA was classified according to the Kellgren-Lawrence grading system. Results Elevated concentrations of fractalkine in serum were found in knee OA patients compared with healthy controls [all results median (interquartile range) 226.25 (183.19-259.91) vs. 127.42 (99.54-154.98)pg/mL, P<0.001]. The case group included 71 knee OA patients with grade 2, 98 with grade 3, and 54 with grade 4. Knee OA patients with KL grade 4 had significantly higher fractalkine concentrations in serum and SF compared with those with KL grade 2 and 3 [serum: 247.68 (215.05-278.64) vs. 212.45 (169.19-247.96)pg/mL, P<0.001, and 247.68 (215.05-278.64) vs. 222.00 (179.80-254.98)pg/mL, P=0.005, respectively; SF: 94.95 (76.46-106.68) vs. 74.31 (63.64-84.79)pg/mL, P<0.001, and 94.95 (76.46-106.68) vs. 80.34 (68.84-96.39)pg/mL, P=0.001, respectively]. Knee OA patients with KL grade 3 showed significantly elevated concentrations of fractalkine in SF compared with those with KL grade 2 [80.34 (68.84-96.39) vs. 74.31 (63.64-84.79)pg/mL, P=0.004]. Fractalkine concentrations in serum and SF of knee OA patients were both significantly associated with the disease severity evaluated by KL grading criteria (r=0.261, P<0.001 and r=0.366, P<0.001, respectively). Conclusion The fractalkine concentrations in serum and SF may serve as an effective biomarker for the severity of OA.
引用
收藏
页码：571 / 575
页数：5
相关论文
共 20 条
[1]   CD16+ monocytes produce IL-6, CCL2, and matrix metalloproteinase-9 upon interaction with CX3CL1-expressing endothelial cells [J].
Ancuta, Petronela ;
Wang, Jianbin ;
Gabuzda, Dana .
JOURNAL OF LEUKOCYTE BIOLOGY, 2006, 80 (05) :1156-1164
[2]   A new class of membrane-bound chemokine with a CX(3)C motif [J].
Bazan, JF ;
Bacon, KB ;
Hardiman, G ;
Wang, W ;
Soo, K ;
Rossi, D ;
Greaves, DR ;
Zlotnik, A ;
Schall, TJ .
NATURE, 1997, 385 (6617) :640-644
[3]  
Blaschke S, 2003, J RHEUMATOL, V30, P1918
[4]  
Bondeson J, 2007, J RHEUMATOL, V34, P523
[5]  
Borzì RM, 2000, ARTHRITIS RHEUM, V43, P1734, DOI 10.1002/1529-0131(200008)43:8<1734::AID-ANR9>3.0.CO
[6]  
2-B
[7]   Chemokines and disease [J].
Gerard, C ;
Rollins, BJ .
NATURE IMMUNOLOGY, 2001, 2 (02) :108-115
[8]   Identification and molecular characterization of fractalkine receptor CX(3)CR1, which mediates both leukocyte migration and adhesion [J].
Imai, T ;
Hieshima, K ;
Haskell, C ;
Baba, M ;
Nagira, M ;
Nishimura, M ;
Kakizaki, M ;
Takagi, S ;
Nomiyama, H ;
Schall, TJ ;
Yoshie, O .
CELL, 1997, 91 (04) :521-530
[9]   RADIOLOGICAL ASSESSMENT OF OSTEO-ARTHROSIS [J].
KELLGREN, JH ;
LAWRENCE, JS .
ANNALS OF THE RHEUMATIC DISEASES, 1957, 16 (04) :494-502
[10]   Fractalkine functions as a chemoattractant for osteoarthritis synovial fibroblasts and stimulates phosphorylation of mitogen-activated protein kinases and Akt [J].
Klosowska, K. ;
Volin, M. V. ;
Huynh, N. ;
Chong, K. K. ;
Halloran, M. M. ;
Woods, J. M. .
CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 2009, 156 (02) :312-319
12