TALENoffer: genome-wide TALEN off-target prediction

被引：69
|
作者：
Grau, Jan [1 ]
Boch, Jens [2 ]
Posch, Stefan [1 ]
机构：
[1] Univ Halle Wittenberg, Inst Comp Sci, D-06099 Halle, Saale, Germany
[2] Univ Halle Wittenberg, Dept Genet, Inst Biol, D-06099 Halle, Saale, Germany
来源：
BIOINFORMATICS | 2013年 / 29卷 / 22期
关键词：
D O I：
10.1093/bioinformatics/btt501
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Transcription activator-like effector nucleases (TALENs) have become an accepted tool for targeted mutagenesis, but undesired off-targets remain an important issue. We present TALENoffer, a novel tool for the genome-wide prediction of TALEN off-targets. We show that TALENoffer successfully predicts known off-targets of engineered TALENs and yields a competitive runtime, scanning complete mammalian genomes within a few minutes.
引用
收藏
页码：2931 / 2932
页数：2
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