Dendrimer-encapsulated copper as a novel oligonucleotides label for sensitive electrochemical stripping detection of DNA hybridization

被引:23
|
作者
Gao, Huan [1 ]
Jiang, Xue [1 ]
Dong, Yang-Jun [1 ]
Tang, Wan-Xin [1 ]
Hou, Cong [1 ]
Zhu, Ning-Ning [1 ]
机构
[1] Shanghai Normal Univ, Coll Life & Environm Sci, Dept Chem, Shanghai 200234, Peoples R China
来源
BIOSENSORS & BIOELECTRONICS | 2013年 / 48卷
关键词
Dendrimer-encapsulated copper; Electrochemical; DNA hybridization; Anodic stripping analysis; METHYLENE-BLUE; NANOPARTICLES; GOLD; AMPLIFICATION; NANOCLUSTERS; ELECTRODES; REDUCTION; NANOTUBES; BIOSENSOR; INDICATOR;
D O I
10.1016/j.bios.2013.04.021
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
This paper describes the synthesis and characterization of a novel electrochemical label for sensitive electrochemical stripping detection of DNA hybridization based on dendrimer-encapsulated copper. The generation 4.5 (G 4.5) carboxyl-terminated poly(amidoamine) dendrimer with a trimesyl core was used as a template for synthesis of Cu2+/dendrimer nanocomposites (Cu-DNCs). Ratios of Cu2+/dendrimer were optimized in order to obtain stable nanocomposites with maximal copper loading in the interior of a polymeric shell. Cu-DNCs labeled DNA probe was employed for determining a target ssDNA immobilized on multi-walled carbon nanotubes-modified glassy carbon electrode (GCE) based on a specific hybridization reaction. The hybridization events were monitored by electrochemical detection of Cu anchored on the hybrids after the release in a diluted nitric acid by anodic stripping differential pulse voltammetry (ASDPV). The results showed that only a complementary sequence could form a dsDNA with the Cu-DNCs DNA probe and give an obvious electrochemical signal. The non-complementary sequence exhibited negligible signal change compared with the blank measurement (means: the electrode containing no target DNA incubating in hybridization buffer solution containing Cu-DNCs DNA probe for a certain time). The use of Cu encapsulated-dendrimer as tags and ASDPV for the detection of the released Cu ions could enhance the hybridization signal, and result in the increase of the sensitivity for the target DNA. Under the conditions employed here, the detection limit for measuring the full complementary sequence is down to pM level. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:210 / 215
页数:6
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