Repression of the steroidogenic acute regulatory gene by the multifunctional transcription factor Yin Yang 1

被引:29
|
作者
Nackley, AC
Shea-Eaton, W
Lopez, D
McLean, MP
机构
[1] Univ S Florida, Dept Obstet & Gynecol, Tampa, FL 33606 USA
[2] Univ S Florida, Dept Biochem & Mol Biol, Tampa, FL 33606 USA
关键词
D O I
10.1210/en.143.3.1085
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The transport of cholesterol to the inner mitochondrial membrane by the steroidogenic acute regulatory (StAR) protein is a critical step in steroidogenesis. The current study was designed to examine whether the multifunctional transcription factor Yin Yang I (YY1) was able to repress activation of the StAR gene. YY1 bound to three putative YY1-binding sites in the rat StAR promoter. Cotransfection of the StAR promoter linked to a luciferase reporter gene and YY1 in the presence or absence of sterol regulatory element binding protein-la (SREBP-1a) resulted in transcriptional repression. YY1 was found to colocalize in the nucleus with SREPB-1a. YY1 inhibited SREBP-1a/nuclear factor Y (NF-Y) enhancement of StAR activation and YY1 decreased SREBP-la binding to a sterol regulatory element in the presence or absence of NF-Y. YY1 also decreased NF-Y binding to a nonconsensus NF-Y-binding motif in the StAR promoter. These studies provide novel information on the mechanisms of YY1-mediated repression of the StAR gene.
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页码:1085 / 1096
页数:12
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