Arid2-IR promotes NF-κB-mediated renal inflammation by targeting NLRC5 transcription

Increasing evidence shows that long non-coding RNAs (lncRNAs) play an important role in a variety of disorders including kidney diseases. It is well recognized that inflammation is the initial step of kidney injury and is largely mediated by nuclear factor Kappa B (NF-kappa B) signaling. We had previously identified lncRNA-Arid2-IR is an inflammatory lncRNA associated with NF-kappa B-mediated renal injury. In this study, we examined the regulatory mechanism through which Arid2-IR activates NF-kappa B signaling. We found that Arid2-IR was differentially expressed in response to various kidney injuries and was induced by transforming growth factor beta 1(TGF-beta 1). Using RNA sequencing and luciferase assays, we found that Arid2-IR regulated the activity of NF-kappa B signal via NLRC5-dependent mechanism. Arid2-IR masked the promoter motifs of NLRC5 to inhibit its transcription. In addition, during inflammatory response, Filamin A (Flna) was increased and functioned to trap Arid2-IR in cytoplasm, thereby preventing its nuclear translocation and inhibition of NLRC5 transcription. Thus, lncRNA Arid2-IR mediates NF-kappa B-driven renal inflammation via a NLRC5-dependent mechanism and targeting Arid2-IR may be a novel therapeutic strategy for inflammatory diseases in general.