Plant proteins, minerals and trace elements of Eurycoma longifolia (Tongkat Ali)

被引：7
作者：
Chua, Lee Suan [1 ]
Abdul-Rahman, Nurulaini [1 ]
Rosidi, Bustanur [1 ]
Lee, Chew Tin [1 ]
机构：
[1] Univ Teknol Malaysia, Inst Bioprod Dev, Metabolites Profiling Lab, Utm Skudai 81310, Johor, Malaysia
来源：
NATURAL PRODUCT RESEARCH | 2013年 / 27卷 / 4-5期
关键词：
Eurycoma longifolia; SDS-PAGE; plant protein; amino acids; metals; 2-DIMENSIONAL GEL-ELECTROPHORESIS; QUASSINOIDS; ROOTS;
D O I：
10.1080/14786419.2012.676552
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A water extraction method has been used to extract plant proteins from the roots of Eurycoma longifolia harvested from Perak and Pahang, Malaysia. On the basis of the spectroscopic Bradford assay, Tongkat Ali Perak and Pahang contained 0.3868 and 0.9573mgmL(1) of crude protein, respectively. The crude proteins were separated by one dimensional 15% sodium dodecyl sulphate polyacrylamide gel electrophoresis into two (49.8 and 5.5kD) and four (49.8, 24.7, 21.1 and 5.5kD) protein spots for Tongkat Ali Perak and Pahang, respectively. Isoleucine was present in the highest concentration significantly. Both plant samples showed differences in the mineral and trace element profiles, but the minerals calcium, magnesium and potassium were present in the highest concentration. The highly concerned toxic metals such as arsenic and lead were not detected.
引用
收藏
页码：314 / 318
页数：5
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