Genetic evidence for the existence of two quinone related inhibitor binding sites in NADH-CoQ reductase

被引：26

作者：

Darrouzet, E ^{[1
]}

Dupuis, A ^{[1
]}

机构：

[1] CEA, DEPT BIOL MOL & STRUCT, LAB BIOENERGET CELLULAIRE & PATHOL, BECP, F-38054 GRENOBLE 09, FRANCE

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 1997年 / 1319卷 / 01期

关键词：

Complex I; NADH-CoQ reductase; piericidin; quinone binding site; Rhodobacter capsulatus;

D O I：

10.1016/S0005-2728(97)00009-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using the NADH-CoQ reductase of Rhodobacter capsulatus as a model for the mitochondrial Complex I, we have for the first time isolated bacterial mutants resistant to piericidin-A, a classical inhibitor of the mitochondrial enzyme. Their sensitivity to other inhibitors directed towards the quinone binding domain of complex I gives direct genetic evidence for the existence of two inhibitor binding sites. (C) 1977 Elsevier Science B.V.

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页码：1 / 4

页数：4

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