Study for Rapid Determination of Tetrodotoxin Based on Lateral Flow Immunoassay

被引：0

作者：

Chen, Jun ^{[1
]}

Zhao, Jing ^{[3
]}

Tao, Ning-Ping ^{[3
]}

Cong, Jian ^{[2
]}

机构：

[1] Shanghai 4 Reagent & H Chenm Co LtD, Shanghai 200940, Peoples R China

[2] Shanghai Open Univ, Coll Urban Publ Secur Adm, Shanghai 200433, Peoples R China

[3] Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

来源：

PROCEEDINGS OF THE 2ND ANNUAL INTERNATIONAL CONFERENCE ON ADVANCED MATERIAL ENGINEERING (AME 2016) | 2016年 / 85卷

关键词：

Tetrodotoxin; Lateral flow immunoassay; Superparamagnetic nanoparticle probe; Rapid detection; ELISA;

D O I：

暂无

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Trichloroacetic acid (TCA) was used as a protein precipitation in the pretreatment process. The content of TCA in the sample was optimized. Lateral flow immunoassay (LFIA) based on superparamagnetic nanoparticle (SPMNP) probe for TTX detection was employed as a detection method. This article is intended to establish a fast, easy, effective TTX detection method and a matching pretreatment method that could be applied to on-site test. It is found that the obtained results were identical with the reference analytical values after the sample treated by 12% TCA and with 5:1:1 ratio of 10% Tween-20 in BS to 2g/mL BSA in BS to 0.35g/mL sucrose in BS combined. The detection limit of TTX-spiked samples was found to be 6 ng/mL. The whole assay time of the qualitative test for TTX detection was less than 1.5h.

引用

页码：1305 / 1314

页数：10

共 13 条

[1]

[Anonymous], 2008, THESIS

[2]

Gong HZ, 2005, CHINESE J PUBLIC HLT, V12, P1423

[3] Comparison of protein precipitation methods for sample preparation prior to proteomic analysis [J].

Jiang, L ;

He, L ;

Fountoulakis, M .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1023 (02) :317-320

[4] Lateral-flow colloidal gold-based immunoassay for the rapid detection of deoxynivalenol with two indicator ranges [J].

Kolosova, Anna Yu. ;

Sibanda, Liberty ;

Dumoulin, Frederic ;

Lewis, Janet ;

Duveiller, Etienne ;

Van Peteghem, Carlos ;

De Saeger, Sarah .

ANALYTICA CHIMICA ACTA, 2008, 616 (02) :235-244

[5]

Ling TT, 2012, JIANGSU AGR SCI, V40, P298

[6] Membrane assisted isoform immunoassay -: A rapid method for the separation and determination of protein isoforms in an integrated immunoassay [J].

Lönnberg, M ;

Carlsson, J .

JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 246 (1-2) :25-36

[7] Magnetic nanogold microspheres-based lateral-flow immunodipstick for rapid detection of aflatoxin B2 in food [J].

Tang, D. ;

Sauceda, J. C. ;

Lin, Z. ;

Ott, S. ;

Basova, E. ;

Goryacheva, I. ;

Biselli, S. ;

Lin, J. ;

Niessner, R. ;

Knopp, D. .

BIOSENSORS & BIOELECTRONICS, 2009, 25 (02) :514-518

[8] Performance of ELISA for specific measurement of High-Molecular-Weight (HMW) adiponectin [J].

Tanita, Toshimi ;

Miyakoshi, Hideo ;

Nakano, Yasuko .

JOURNAL OF IMMUNOLOGICAL METHODS, 2008, 333 (1-2) :139-146

[9] Robotic sample pretreatment immunoassay determination of chlorpyrifos metabolite (TCP) in soil and fruit [J].

VelascoArjona, A ;

Manclus, JJ ;

Montoya, A ;

deCastro, MDL .

TALANTA, 1997, 45 (02) :371-377

[10]

Wu SJ, 2005, MAR SCI, V10, P83

← 1 2 →