Ras-related proteins (Rab) are key proteins related to male fertility following a unique activation mechanism

被引：35

作者：

Bae, Jeong-Won ^{[1
]}

Kim, So-Hye ^{[1
]}

Kim, Dae-Hyun ^{[2
]}

Ha, Jae Jung ^{[2
]}

Yi, Jun Koo ^{[2
]}

Hwang, Seongsoo ^{[3
]}

Ryu, Buom-Yong ^{[4
]}

Pang, Myung-Geol ^{[4
]}

Kwon, Woo-Sung ^{[1
,5
]}

机构：

[1] Kyungpook Natl Univ, Dept Anim Sci & Biotechnol, Sangju 37224, Gyeongsangbuk D, South Korea

[2] Gyeongbuk Livestock Res Inst, Yeongju 36052, Gyeongsangbuk D, South Korea

[3] Natl Inst Anim Sci, RDA, Anim Biotechnol Div, Wanju Gun 55365, Jeollabuk Do, South Korea

[4] Chung Ang Univ, Dept Anim Sci & Technol, Anseong 456756, Gyeonggi Do, South Korea

[5] Kyungpook Natl Univ, Dept Anim Biotechnol, Sangju 37224, Gyeongsangbuk D, South Korea

来源：

REPRODUCTIVE BIOLOGY | 2019年 / 19卷 / 04期

关键词：

Rab proteins; Spermatozoa; Capacitation; Acrosome reaction; Male fertility; GDP DISSOCIATION INHIBITOR; POSTTRANSLATIONAL MODIFICATIONS; GTPASES; ORGANIZATION; SPERM; TRIGGERS;

D O I：

10.1016/j.repbio.2019.10.001

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ras-related protein Rab (Rab) proteins, member of Ras superfamily of monomeric G proteins, are well known key regulators of intracellular vesicular transport. Recently, it has been reported that Rab 2A and 3A are related to acrosomal exocytosis in spermatozoa and Rab 2A can be used to fertility-related biomarker in male. However, the role and mechanism of Rab proteins in spermatozoa has not been fully understood yet. Therefore, the study to analyze the expression and location of various Rab proteins in spermatozoa is required to understand the role and mechanism of Rab proteins in spermatozoa. In present study, to analyze the expression level and location of various Rab proteins (Rab 2A, Rab3A, Rab4, Rab5, Rab8A, Rab9, Rab11, Rab14, Rab25, Rab27A, and Rab34) and Rab protein regulators (RabGAP, RabGDI, RabGEF) in spermatozoa following capacitation, immunofluorescence and western blot analysis were performed. All of 11 Rab proteins were expressed in acrosomal region and tail of spermatozoa. Furthermore, all Rab proteins and Rab protein regulators, except RabGAP, have decreased expression patterns after capacitation. Taken together, Rab proteins were located in sperm head and tail. In addition, expression patterns of Rab proteins in spermatozoa were altered following capacitation. Therefore, our results suggested that Rab proteins may be key proteins related with capacitation as well as playing important role with uniquely activation pathway for male fertility.

引用

页码：356 / 362

页数：7

共 30 条

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