Single Cell Oil Producing Yeasts Lipomyces starkeyi and Rhodosporidium toruloides: Selection of Extraction Strategies and Biodiesel Property Prediction

被引：63

作者：

Bonturi, Nemailla ^{[1
,2
]}

Matsakas, Leonidas ^{[2
]}

Nilsson, Robert ^{[2
]}

Christakopoulos, Paul ^{[2
]}

Miranda, Everson Alves ^{[1
]}

Berglund, Kris Arvid ^{[2
,3
]}

Rova, Ulrika ^{[2
]}

机构：

[1] Univ Estadual Campinas, Sch Chem Engn, Dept Mat & Bioproc Engn, BR-13083852 Campinas, SP, Brazil

[2] Lulea Univ Technol, Dept Civil Environm & Nat Resources, Div Chem Engn, Biochem Proc Engn, SE-97187 Lulea, Sweden

[3] Michigan State Univ, Dept Chem Engn & Mat Sci, E Lansing, MI 48824 USA

来源：

ENERGIES | 2015年 / 8卷 / 06期

基金：

巴西圣保罗研究基金会;

关键词：

MICROBIAL LIPID PRODUCTION; FATTY-ACID-COMPOSITION; OLEAGINOUS YEAST; RAPID METHOD; ACCUMULATION; PURIFICATION; CULTIVATION; CULTURE;

D O I：

10.3390/en8065040

中图分类号：

TE [石油、天然气工业]; TK [能源与动力工程];

学科分类号：

0807 ; 0820 ;

摘要：

Single cell oils (SCOs) are considered potential raw material for the production of biodiesel. Rhodosporidium sp. and Lipomyces sp. are good candidates for SCO production. Lipid extractability differs according to yeast species and literature on the most suitable method for each oleaginous yeast species is scarce. This work aimed to investigate the efficiency of the most cited strategies for extracting lipids from intact and pretreated cells of Rhodosporidium toruloides and Lipomyces starkeyi. Lipid extractions were conducted using hexane or combinations of chloroform and methanol. The Folch method resulted in the highest lipid yields for both yeasts (42% for R. toruloides and 48% for L. starkeyi). Also, this method eliminates the cell pretreatment step. The Bligh and Dyer method underestimated the lipid content in the tested strains (25% for R. toruloides and 34% for L. starkeyi). Lipid extractability increased after acid pretreatment for the Pedersen, hexane, and Bligh and Dyer methods. For R. toruloides unexpected fatty acid methyl esters (FAME) composition were found for some lipid extraction strategies tested. Therefore, this work provides useful information for analytical and process development aiming at biodiesel production from the SCO of these two yeast species.

引用

页码：5040 / 5052

页数：13

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[1] Oily yeasts as oleaginous cell factories [J].