PEDF from mouse mesenchymal stem cell secretome attracts fibroblasts

被引：50

作者：

Sarojini, Harshini ^{[2
]}

Estrada, Rosendo ^{[2
]}

Lu, Hongwei ^{[2
]}

Dekova, Silvia ^{[2
]}

Lee, Menq-Jer ^{[2
,3
]}

Gray, Robert D. ^{[1
,2
]}

Wang, Eugenia ^{[1
,2
]}

机构：

[1] Univ Louisville, Sch Med, Dept Biochem & Mol Biol, Louisville, KY 40202 USA

[2] Univ Louisville, Sch Med, Gheens Ctr Aging, Louisville, KY 40202 USA

[3] Univ Louisville, Sch Med, Dept Microbiol & Immunol, Louisville, KY 40202 USA

来源：

JOURNAL OF CELLULAR BIOCHEMISTRY | 2008年 / 104卷 / 05期

关键词：

murine marrow stromal cells; secretome; fibroblast chemoattractant; PEDF;

D O I：

10.1002/jcb.21748

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conditioned medium (secretome) derived from an enriched stein cell culture stimulates chemotaxis of human fibroblasts. These cells are classified as multipotent murine mesenchymal stromal cells (mMSC) by immunochemical analysis of marker proteins. Proteomic analysis of mMSC secretome identifies nineteen secreted proteins, including extracellular matrix structural proteins, collagen processing enzymes, pigment epithelium-derived factor (PEDF) and cystatin C. Immunodepletion and reconstitution experiments show that PEDF is the predominant fibroblast chemoattractant in the conditioned medium, and immunofluorescence microscopy shows strong staining for PEDF in the cytoplasm, at the cell surface, and in intercellular space between mMSCs. This stimulatory effect of PEDF on fibroblast chemotaxis is in contrast to the PEDF-mediated inhibition of endothelial cell migration, reported previously. These differential functional effects of PEDF toward fibroblasts and endothelial cells may serve to program an ordered temporal sequence of scaffold building followed by angiogenesis during Wound healing. J. Cell. Biochem. 104: 1793-1802, 2008. (C) 2008 Wiley-Liss, Inc.

引用

页码：1793 / 1802

页数：10

共 28 条

[21] Pigment epithelium-derived factor is a niche signal for neural stem cell renewal [J].

Ramírez-Castillejo, C ;

Sánchez-Sánchez, F ;

Andreu-Agulló, C ;

Ferrón, SR ;

Aroca-Aguilar, JD ;

Sánchez, P ;

Mira, H ;

Escribano, J ;

Fariñas, I .

NATURE NEUROSCIENCE, 2006, 9 (03) :331-339

[22] The stem-cell niche as an entity of action [J].

Scadden, DT .

NATURE, 2006, 441 (7097) :1075-1079

[23]

SCHOFIELD R, 1978, BLOOD CELLS, V4, P7

[24] PIGMENT EPITHELIUM-DERIVED FACTOR - NEUROTROPHIC ACTIVITY AND IDENTIFICATION AS A MEMBER OF THE SERINE PROTEASE INHIBITOR GENE FAMILY [J].

STEELE, FR ;

CHADER, GJ ;

JOHNSON, LV ;

TOMBRANTINK, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (04) :1526-1530

[25] Isolation of mouse marrow mesenchymal progenitors by a novel and reliable method [J].

Sun, SK ;

Guo, ZK ;

Xiao, XR ;

Liu, B ;

Liu, XD ;

Tang, PH ;

Mao, N .

STEM CELLS, 2003, 21 (05) :527-535

[26] PEDF: A multifaceted neurotrophic factor [J].

Tombran-Tink, J ;

Barnstable, CJ .

NATURE REVIEWS NEUROSCIENCE, 2003, 4 (08) :628-636

[27] Translating stem and progenitor cell biology to the clinic: Barriers and opportunities [J].

Weissman, IL .

SCIENCE, 2000, 287 (5457) :1442-1446

[28] Dual-site recognition of different extracellular matrix components by anti-angiogenic/neurotrophic serpin, PEDF [J].

Yasui, N ;

Mori, T ;

Morito, D ;

Matsushita, O ;

Kourai, H ;

Nagata, K ;

Koide, T .

BIOCHEMISTRY, 2003, 42 (11) :3160-3167

← 1 2 3 →