MiRNA-218, a new regulator of HMGB1, suppresses cell migration and invasion in non-small cell lung cancer

被引：71

作者：

Zhang, Cailian ^{[1
]}

Ge, Shengli ^{[2
]}

Hu, Cailian ^{[3
]}

Yang, Ning ^{[1
]}

Zhang, Jinran ^{[1
]}

机构：

[1] Yanan Univ, Affiliated Hosp, Dept Geriatr, Yanan 716000, Peoples R China

[2] Yanan Univ, Affiliated Hosp, Dept Ophthalmol, Yanan 716000, Peoples R China

[3] Yanan Univ, Affiliated Hosp, Dept Pulm Med, Yanan 716000, Peoples R China

来源：

ACTA BIOCHIMICA ET BIOPHYSICA SINICA | 2013年 / 45卷 / 12期

关键词：

microRNA; miR-218; non-small lung cancer; HMGB1; TUMOR-SUPPRESSOR; HEPATOCELLULAR-CARCINOMA; ADP-RIBOSYLATION; BREAST-CANCER; INTACT-CELLS; EXPRESSION; MICRORNA; GENES; PROTEIN; INVASIVENESS;

D O I：

10.1093/abbs/gmt109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRNAs) function as negative regulators of gene expression involved in cancer metastasis. The aim of this study is to investigate the potential roles of miR-218 in non-small cell lung cancer and validate its regulation mechanism. Functional studies showed that miR-218 overexpression inhibited cell migration and invasion, but had no effect on cell viability. Enhanced green fluorescent protein reporter assay, real-time polymerase chain reaction and western blot analysis confirmed that miR-218 suppressed the expression of high mobility group box-1 (HMGB1) by directly targeting its 3'-untranslated region. Accordingly, silencing of HMGB1 accorded with the effects of miR-218 on cell migration and invasion, and overexpression of HMGB1 can restore cell migration and invasion which were reduced by miR-218. In conclusion, these findings demonstrate that miR-218 functions as a tumor suppressor in lung cancer. Furthermore, miR-218 may act as a potential therapeutic biomarker for metastatic lung cancer patients.

引用

页码：1055 / 1061

页数：7

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