Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato

被引：32

作者：

Just, Daniel ^{[1
,2
]}

Garcia, Virginie ^{[1
,2
]}

Fernandez, Lucie ^{[1
,2
]}

Bres, Cecile ^{[1
,2
]}

Mauxion, Jean-Philippe ^{[1
,2
]}

Petit, Johann ^{[1
,2
]}

Jorly, Joana ^{[1
,2
]}

Assali, Julien ^{[1
,2
]}

Bournonville, Celine ^{[1
,2
]}

Ferrand, Carine ^{[1
,2
]}

Baldet, Pierre ^{[1
,2
]}

Lemaire-Chamley, Martine ^{[1
,2
]}

Mori, Kentaro ^{[1
,2
,3
]}

Okabe, Yoshihiro ^{[3
]}

Ariizumi, Tohru ^{[3
]}

Asamizu, Erika ^{[3
]}

Ezura, Hiroshi ^{[3
]}

Rothan, Christophe ^{[1
,2
]}

机构：

[1] INRA Bordeaux, UMR Biol Fruit & Pathol 1332, F-33140 Villenave Dornon, France

[2] Univ Bordeaux, UMR 1332, F-33140 Villenave Dornon, France

[3] Univ Tsukuba, Grad Sch Life & Environm Sci, Ctr Gene Res, Tsukuba, Ibaraki 3058572, Japan

来源：

PLANT BIOTECHNOLOGY | 2013年 / 30卷 / 03期

关键词：

Tomato; mutants; TILLING; forward genetics; deep sequencing; phenotype; database; MODEL SYSTEM; PROVIDES INSIGHTS; INDUCED MUTATIONS; REVERSE GENETICS; POINT MUTATIONS; CANDIDATE GENES; FRUIT; IDENTIFICATION; EXPRESSION; ENDOREDUPLICATION;

D O I：

10.5511/plantbiotechnology.13.0622a

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Tomato is currently the model plant for fleshy fruit development and for Solanaceae species. Recent genomic approaches including transcriptome, proteome and metabolome analyses and genetic mapping have produced a wealth of candidate genes whose function needs to be assessed. The recent development in model and crop plants of TILLING (Targeting Induced Local Lesions IN Genomes), which reveals allelic series corresponding to several independent point mutations, and the current availability of deep sequencing tools further increase the interest of generating artificially-induced genetic diversity in tomato. We describe here the generation and use of EMS (ethyl methanesulfonate) tomato mutants in the miniature cultivar Micro-Tom and provide as example the identification of new fruit size and morphology mutants. We further propose new deep sequencing-based strategies for the discovery of mutations underlying phenotypic variations observed in mutant collections that will considerably increase the interest of exploiting Micro-Tom mutant collections for gene discovery in tomato.

引用

页码：225 / 231

页数：7

共 53 条

[1] Genome sequencing reveals agronomically important loci in rice using MutMap [J].