Analysis of monosaccharide composition of Cyclocarya paliurus polysaccharide with anion exchange chromatography

被引：123
作者：
Xie, Jian-Hua [1 ]
Shen, Ming-Yue [1 ]
Nie, Shao-Ping [1 ]
Liu, Xin [1 ]
Zhang, Hui [1 ]
Xie, Ming-Yong [1 ]
机构：
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2013年 / 98卷 / 01期
基金：
中国国家自然科学基金;
关键词：
Cyclocarya paliurus (Batal.) Iljinskaja; Polysaccharide; High-performance anion-exchange chromatography; Monosaccharide composition; PULSED-AMPEROMETRIC DETECTION; PERFORMANCE LIQUID-CHROMATOGRAPHY; BATAL; ILJINSKAJA; ANTIOXIDANT ACTIVITIES; ASSISTED EXTRACTION; GAS-CHROMATOGRAPHY; ELECTROPHORESIS; SEPARATION; ELEMENTS; LEAVES;
D O I：
10.1016/j.carbpol.2013.07.011
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
A simple, rapid and sensitive analytical method for the determination of monosaccharide composition in Cyclocarya paliurus polysaccharide was developed and validated. This method was based on hydrolysis of the polysaccharides followed by high-performance anion-exchange chromatography analysis. The effects of sodium hydroxide concentration and column temperature on retention and separation of the monosaccharide were investigated with Carbo PAC (TM) PA10 analytical column. The established method was validated and the results showed that this method had good linearity (R-2,0.9987-0.9999), adequate accuracy (98.53-102.13% recovery), high precision (relative standard deviation <3.8%) and sensitive detection limits (2.57-7.86 nM), with a simple preparation of the samples (no need to derivatize the samples) and short run time (20 min). The results showed that the C paliurus polysaccharide consisted of rhamnose, arabinose, galactose, glucose, mannose and xylose in the molar ratio of 1.00:1.85:3.26:3.12:0.85:0.29. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：976 / 981
页数：6
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