Analysis of monosaccharide composition of Cyclocarya paliurus polysaccharide with anion exchange chromatography

被引:123
作者
Xie, Jian-Hua [1 ]
Shen, Ming-Yue [1 ]
Nie, Shao-Ping [1 ]
Liu, Xin [1 ]
Zhang, Hui [1 ]
Xie, Ming-Yong [1 ]
机构
[1] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Peoples R China
基金
中国国家自然科学基金;
关键词
Cyclocarya paliurus (Batal.) Iljinskaja; Polysaccharide; High-performance anion-exchange chromatography; Monosaccharide composition; PULSED-AMPEROMETRIC DETECTION; PERFORMANCE LIQUID-CHROMATOGRAPHY; BATAL; ILJINSKAJA; ANTIOXIDANT ACTIVITIES; ASSISTED EXTRACTION; GAS-CHROMATOGRAPHY; ELECTROPHORESIS; SEPARATION; ELEMENTS; LEAVES;
D O I
10.1016/j.carbpol.2013.07.011
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
A simple, rapid and sensitive analytical method for the determination of monosaccharide composition in Cyclocarya paliurus polysaccharide was developed and validated. This method was based on hydrolysis of the polysaccharides followed by high-performance anion-exchange chromatography analysis. The effects of sodium hydroxide concentration and column temperature on retention and separation of the monosaccharide were investigated with Carbo PAC (TM) PA10 analytical column. The established method was validated and the results showed that this method had good linearity (R-2,0.9987-0.9999), adequate accuracy (98.53-102.13% recovery), high precision (relative standard deviation <3.8%) and sensitive detection limits (2.57-7.86 nM), with a simple preparation of the samples (no need to derivatize the samples) and short run time (20 min). The results showed that the C paliurus polysaccharide consisted of rhamnose, arabinose, galactose, glucose, mannose and xylose in the molar ratio of 1.00:1.85:3.26:3.12:0.85:0.29. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码:976 / 981
页数:6
相关论文
共 32 条
[1]   Quantitative Prediction of Cell Wall Polysaccharide Composition in Grape (Vitis vinifera L.) and Apple (Malus domestica) Skins from Acid Hydrolysis Monosaccharide Profiles [J].
Arnous, Anis ;
Meyer, Anne S. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (09) :3611-3619
[2]   SEPARATION AND IDENTIFICATION OF ACID POLYSACCHARIDES BY THIN-LAYER CHROMATOGRAPHY [J].
BISCHEL, MD ;
AUSTIN, JH ;
KEMENY, MD ;
HUBBLE, CM ;
LEAR, RK .
JOURNAL OF CHROMATOGRAPHY, 1966, 21 (01) :40-&
[3]   Determination of saccharides in atmospheric aerosol using anion-exchange high-performance liquid chromatography and pulsed-amperometric detection [J].
Caseiro, Alexandre ;
Marr, Iain L. ;
Claeys, Magda ;
Kasper-Giebl, Anne ;
Puxbaum, Hans ;
Pio, Casimiro A. .
JOURNAL OF CHROMATOGRAPHY A, 2007, 1171 (1-2) :37-45
[4]   Analysis of the Monosaccharide Composition of Purified Polysaccharides in Ganoderma atrum by Capillary Gas Chromatography [J].
Chen, Yi ;
Xie, Ming-Yong ;
Wang, Yuan-Xing ;
Nie, Shao-Ping ;
Li, Chang .
PHYTOCHEMICAL ANALYSIS, 2009, 20 (06) :503-510
[5]   Simultaneous analyses of neutral carbohydrates and amino sugars in freshwaters with HPLC-PAD [J].
Cheng, XH ;
Kaplan, LA .
JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2003, 41 (08) :434-438
[6]  
Corradini Claudio, 2012, International Journal of Carbohydrate Chemistry, DOI 10.1155/2012/487564
[7]   Sugar compositional determination of polysaccharides from Dunaliella salina by modified RP-HPLC method of precolumn derivatization with 1-phenyl-3-methyl-5-pyrazolone [J].
Dai, Jun ;
Wu, Yan ;
Chen, Shang-wei ;
Zhu, Song ;
Yin, Hong-ping ;
Wang, Min ;
Tang, Jian .
CARBOHYDRATE POLYMERS, 2010, 82 (03) :629-635
[8]   Quantitative determination of wine polysaccharides by gas chromatography-mass spectrometry (GC-MS) and size exclusion chromatography (SEC) [J].
Guadalupe, Zenaida ;
Martinez-Pinilla, Olga ;
Garrido, Alvaro ;
David Carrillo, Jose ;
Ayestaran, Belen .
FOOD CHEMISTRY, 2012, 131 (01) :367-374
[9]   Analysis of monosaccharide composition by capillary electrophoresis [J].
Guttman, A .
JOURNAL OF CHROMATOGRAPHY A, 1997, 763 (1-2) :271-277
[10]  
Holmbom B., 1999, PULPING PAPERMAKING