Characterization and cellular interaction of fluorescent-labeled PHEMA nanoparticles

被引：6

作者：

Gokturk, Ilgim ^{[1
,2
]}

Karakoc, Veyis ^{[2
]}

Onur, Mehmet Ali ^{[3
]}

Denizli, Adil ^{[2
]}

机构：

[1] Hacettepe Univ, Dept Nanotechnol & Nanomed, Ankara, Turkey

[2] Hacettepe Univ, Dept Chem, Div Biochem, TR-06532 Ankara, Turkey

[3] Hacettepe Univ, Dept Biol, Ankara, Turkey

来源：

ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY | 2013年 / 41卷 / 02期

关键词：

acridine orange; cell labeling; miniemulsion polymerization; polymeric nanoparticles; MINIEMULSION TECHNIQUE; POLYMERIC PARTICLES; CELLS; NANOCAPSULES; PURIFICATION; MICROSCOPY; PROBES;

D O I：

10.3109/21691401.2012.742099

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of this study is to label the cells with polymeric nanoparticles properly and efficiently. For this purpose, acridine orange (AO)-loaded poly(2-hydroxyethyl methacrylate) (PHEMA) nanoparticles were synthesized by miniemulsion polymerization. PHEMA nanoparticles were characterized by zeta sizer, zeta potential, atomic force microscopy (AFM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), fluorescence microscopy, Fourier transform infrared spectrophotometer (FTIR), and elemental analysis. In addition, the toxicity of the nanoparticles were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay.

引用

页码：78 / 84

页数：7

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