Correlation of synovial tissue protein abundance with menopause in osteoarthritis

Background: Osteoarthritis (OA) is a common articular disorder. Epidemiologic surveys show a higher prevalence of OA in women than men and that morbidity is higher during menopause. We aimed to explore whether menopause influences the clinical recovery of a knee joint following OA and injury, and identify associated mecha-nisms by analyzing the proteomic profile of synovial tissue (ST) samples. Methods: Routine blood examination and hormone level tests were conducted before surgery. ST samples from eight participants were collected intraopera-tively for proteomic analysis. One day before and one month after the surgery, we assessed various aspects of func-tion in the affected knee including the with Visual Analog Score (VAS), Lysholm, The Western Ontario, and McMaster Universities Osteoarthritis Index (WOMAC) scores. The relationships between proteomic data, estrogen levels, and affected knee function were compared and analyzed. This was a retrospective study. Result: Menopause was as-sociated with the clinical outcomes of knee OA and knee injuries. ST proteomic data identified that 80 proteins in premenopausal OA females were significantly different from menopausal OA females. In addition, 100 proteins were significantly different between premenopausal OA females and premenopausal injured females. Conclusions: Age and menopause showed a positive correlation with the protein profile of ST from OA or knee injury female patients, indicating that the protein components might be affected by menopause. Postoperative clinical outcomes were af-fected by menopause. We conclude that menopause may, in part, regulate knee joint function by altering ST protein expression.