Fusion of Enhanced Green Fluorescent Protein to the Pseudorabies Virus Axonal Sorting Protein Us9 Blocks Anterograde Spread of Infection in Mammalian Neurons

被引：12

作者：

Lyman, M. G. ^{[1
]}

Curanovic, D. ^{[1
]}

Brideau, A. D. ^{[1
]}

Enquist, L. W. ^{[1
]}

机构：

[1] Princeton Univ, Dept Mol Biol, Schultz Lab 314, Princeton, NJ 08544 USA

来源：

JOURNAL OF VIROLOGY | 2008年 / 82卷 / 20期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1128/JVI.01204-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Pseudorabies virus encodes a membrane protein (Us9) that is essential for the axonal sorting of virus particles within neurons and anterograde spread in the mammalian nervous system. Enhanced green fluorescent protein (GFP)-tagged Us9 mimicked the trafficking properties of the wild-type protein in nonneuronal cells. We constructed a pseudorabies virus strain that expressed Us9-GFP and tested its spread capabilities in the rat visual system and in primary neuronal cultures. We report that Us9-EGFP does not promote anterograde spread of infection and may disrupt packing of viral membrane proteins in lipid rafts, an essential step for Us9-mediated axonal sorting.

引用

页码：10308 / 10311

页数：4

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