Bovine carriers of Anaplasma marginale and Anaplasma bovis in South India

被引：0

作者：

Nair, A. S. ^{[1
]}

Ravindran, R. ^{[2
]}

Lakshmanan, B. ^{[2
]}

Sreekumar, C. ^{[3
]}

Kumar, S. S. ^{[2
]}

Raju, Remya ^{[4
]}

Tresamol, P., V ^{[1
]}

Vimalkumar, M. B. ^{[2
]}

Saseendranath, M. R. ^{[1
]}

机构：

[1] Coll Vet & Anim Sci, Dept Vet Epidemiol & Prevent Med, Trichur, Kerala, India

[2] Coll Vet & Anim Sci, Dept Vet Parasitol, Wayanad 673576, Kerala, India

[3] Tamil Nadu Vet & Anim Sci Univ, Dept Vet Parasitol, Madras, Tamil Nadu, India

[4] Coll Vet & Anim Sci, Dept Stat, Wayanad 673576, Kerala, India

来源：

TROPICAL BIOMEDICINE | 2013年 / 30卷 / 01期

关键词：

CATTLE; EHRLICHIA; PREVALENCE; TICKS; DNA; AGENT;

D O I：

暂无

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Carriers of bovine anaplasmosis in Northern Kerala, South India were detected using conventional microscopical and molecular techniques. PCR-RFLP and nested PCR techniques were used for detection of Anaplasma marginale and Anaplasma bovis respectively and the PCR products were confirmed by sequencing. Out of 150 samples tested, 25 were detected positive for A. marginale and five for A. bovis based on molecular tests. The inclusion bodies of A. marginale could be detected by microscopy in two blood smears after staining by giemsa while acridine orange staining detected three smears positive. The data clearly suggest the higher sensitivity of molecular techniques for diagnosis of these diseases.

引用

页码：105 / 112

页数：8

共 35 条

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