Cleavage of eukaryotic initiation factor eIF5B by enterovirus 3C proteases

被引：73
作者：
de Breyne, Sylvain [1 ]
Bonderoff, Jennifer M. [1 ,2 ]
Chumakov, Konstantin M. [3 ]
Lloyd, Richard E. [1 ]
Hellen, Christopher U. T. [1 ]
机构：
[1] Suny Downstate Med Ctr, Dept Microbiol & Immunol, Brooklyn, NY 11203 USA
[2] Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA
[3] US FDA, Ctr Biol Evaluat & Res, Rockville, MD 20852 USA
来源：
VIROLOGY | 2008年 / 378卷 / 01期
关键词：
coxsackievirus; eIF5B; poliovirus; protease; rhinovirus; translation shutoff;
D O I：
10.1016/j.virol.2008.05.019
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The enteroviruses poliovirus (PV), Coxsackie B virus (CVB) and rhinovirus (HRV) are members of Picorna-viridae that inhibit host cell translation early in infection. Enterovirus translation soon predominates in infected cells, but eventually also shuts off. This complex pattern of modulation of translation suggests regulation by a multifactorial mechanism. We report here that eIF5B is proteolytically cleaved during PV and CVB infection of cultured cells, beginning at 3 hours post-infection and increasing thereafter. Recombinant PV, CVB and HRV 3C(pro) cleaved purified native rabbit eukaryotic initiation factor (eIF) 5B in vitro at a single site (VVEQ down arrow G, equivalent to VMEQ down arrow G(479) in human eIF5B) that is consistent with the cleavage specificity of enterovirus 3C proteases. Cleavage separates the N-terminal domain of eIF5B from its essential conserved central GTPase and C-terminal domains. 3C(pro)-mediated cleavage of eIF5B may thus play an accessory role in the shutoff of translation that occurs in enterovirus-infected cells. (c) 2008 Elsevier Inc. All rights reserved.
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收藏
页码：118 / 122
页数：5
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