A REAL-TIME CELL PROLIFERATION AND MOTILITY MONITORING SYSTEM

被引：0

作者：

Moscelli, Nicola ^{[1
]}

van den Driesche, Sander ^{[1
]}

Vellekoop, Michael J. ^{[1
]}

Witarski, Wojciech ^{[2
]}

机构：

[1] Vienna Univ Technol, Inst Sensors & Actuator Syst, Gusshausstr 27-29,E366, A-1040 Vienna, Austria

[2] Slovak Acad Sci, Inst Virol, Bratislava, Slovakia

来源：

BIODEVICES 2011 | 2011年

关键词：

Optical detection; CCD sensor; Real-time monitoring; Epithelial cells;

D O I：

暂无

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

In this contribution we present a compact imaging system to monitor the proliferation and the motility of cells in real-time. Our monitoring system is compatible with standard multi-well plates and operates in CO2, temperature and humidity controlled cell-culture incubators. Adherent grown epithelial cells in a multi-well plate well, positioned on top of a custom made holder, have been monitored in real-time with a fixed CCD image sensor. As light source an LED is placed above the plate holder. A field of view of 3.3 x 2.5 mm(2) was achieved by using a 4.6 x 4.0 mm(2) image sensor and mini lens system. The image sensor has a resolution of 640 x 480 pixels. Consequently, the obtained sensing resolution of the imaging system is about 5 mu m. The cell monitoring system has first been validated by visualizing micro-beads of known dimensions. Then, our system has been successfully tested, tracking the migration paths and proliferation of respectively adherent grown MDCK (Madin-Darby canine kidney) and A549 (human lung carcinoma) epithelial cells.

引用

页码：230 / +

页数：2