Spectroscopic studies on the interaction between methylene blue and bovine serum albumin

被引:280
作者
Hu, YJ
Liu, Y [1 ]
Zhao, RM
Dong, JX
Qu, SS
机构
[1] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China
[2] Wuhan Univ, State Key Lab Virol, Wuhan 430072, Peoples R China
基金
中国国家自然科学基金;
关键词
methylene blue; bovine serum albumin; fluorescence quenching; thermodynamic parameters; fluorescence resonance energy transfer;
D O I
10.1016/j.jphotochem.2005.08.037
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
The interaction between methylene blue (MB) and bovine serum albumin (BSA) was investigated by fluorescence and UV-vis absorbance spectroscopy. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by ME is mainly a result of the formation of MB-BSA complex and electrostatic interactions play an important role to stabilize the complex. The Stern-Volmer quenching constant K-SV and corresponding thermodynamic parameters Delta H, Delta G, and Delta S were calculated. The distance r between donor (BSA) and acceptor (MB) was obtained according to fluorescence resonance energy transfer (FRET). The effect of MB on the conformation of BSA has been analyzed by means of UV-vis absorbance spectra and synchronous fluorescence spectroscopy. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:324 / 329
页数:6
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