Optimization of Chondroitin Sulfate Extraction from Eggshell Membrane with Response Surface Methodology

被引：0
|
作者：
Liu, Ning [1 ]
Luan, Xin-Yue [1 ]
Li, Chen-Chen [1 ]
Luan, You-Shun [1 ]
Liu, Tao [1 ]
Li, Jian [1 ]
机构：
[1] Harbin Univ Commerce, Coll Food Engn, Key Lab Food Sci & Engn Heilongjiang Prov, Harbin 150076, Heilongjiang, Peoples R China
来源：
2016 INTERNATIONAL CONFERENCE ON MATERIALS SCIENCE AND ENGINEERING APPLICATION (ICMSEA 2016) | 2016年
关键词：
Chondroitin sulfate; Egg shell membrane; Extraction; Response surface methodology;
D O I：
暂无
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Using abandoned eggshell membrane as experimental materials, chondroitin sulfate was extracted through base-salt method. The effect of extraction time, extraction temperature, base-salt ratio and liquid-solid ratio on chondroitin sulfate extraction rate was studied through single factor and response surface methodology. The results indicated the optimal reaction conditions of chondroitin sulfate extraction: extraction time was 8h, extraction temperature was 65 degrees C, base-salt ratio was 6:1(v/v) and liquid-solid ratio was 15:1(v/w), which resulted in an extraction yield of 74.0%. The reliability and feasibility of the optimal reaction conditions were proved by the response surface design tests and verification tests.
引用
收藏
页码：406 / 412
页数：7
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