Histone H4 lysine 16 acetylated isoform synthesis opens new route to biophysical studies

被引：5

作者：

Pandita, Tej K. ^{[1
]}

机构：

[1] Univ Texas SW Med Ctr Dallas, Dept Radiat Oncol, Dallas, TX 75390 USA

来源：

PROTEOMICS | 2013年 / 13卷 / 10-11期

关键词：

Cancer; DNA damage response; H4K16ac; Transcription; H4-K16; ACETYLATION; TAIL ACETYLATIONS; MOF; ACETYLTRANSFERASE; REPAIR; HMOF;

D O I：

10.1002/pmic.201300145

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Histone H4 lysine acetylation regulated by MOF (males absent on the first) was initially discovered as a dosage compensation epigenetic mark. Recent studies have revealed, however, that the epigenetic mark has a critical role in cellular function both during oogenesis as well as oncogenesis. Detailed molecular analysis of H4K16 isoforms and other posttranslational modified histones has been limited by the lack of means to prepare sufficient material for in vitro study. This paper describes an improved method to prepare acetylated H4K16 as well as other covalently modified histone H4 isoform for biophysical studies.

引用

页码：1546 / 1547

页数：2

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