Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase

被引：31

作者：

Matsumoto, Chinami ^{[1
,2
]}

Sekine-Suzuki, Emiko ^{[1
,3
]}

Nyui, Minako ^{[1
]}

Ueno, Megumi ^{[1
]}

Nakanishi, Ikuo ^{[1
]}

Omiya, Yuji ^{[2
]}

Fukutake, Masato ^{[1
,2
]}

Kase, Yoshio ^{[2
]}

Matsumoto, Ken-ichiro ^{[1
]}

机构：

[1] Natl Inst Radiol Sci, Adv Particle Radiat Biol Res Program, Res Ctr Charged Particle Therapy, Radioredox Response Res Team,Inage Ku, Chiba, Chiba 2638555, Japan

[2] Tsumura & Co, Tsumura Res Labs, Ami, Ibaraki 3001192, Japan

[3] Natl Inst Radiol Sci, Res Ctr Charged Particle Therapy, Res Program Applicat Heavy Ions Med Sci, Inage Ku, Chiba, Chiba 2638555, Japan

来源：

JOURNAL OF RADIATION RESEARCH | 2015年 / 56卷 / 04期

关键词：

hangeshashinto (HST); oral mucositis (OM); electron paramagnetic resonance (EPR); radiation; reactive oxygen species; CLINICAL-PRACTICE GUIDELINES; RADICAL-SCAVENGING ACTIVITY; ORAL MUCOSITIS; NITRIC-OXIDE;

D O I：

10.1093/jrr/rrv023

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Oral mucositis (OM) is a common and painful complication of radiotherapy for head and neck cancer. Hangeshashinto (HST), a Japanese traditional medicine, is known to alleviate radiotherapy- and/or chemotherapy-induced OM; however, the detailed mechanism has not yet been clarified. The aim of the present study was to clarify the details of the antioxidative functions of HST against reactive oxygen species (ROS) produced by radiation. The hydroxyl radical (center dot OH)-scavenging ability and the reduction ability was simultaneously measured using a modified electron paramagnetic resonance (EPR) spin-trapping method. The superoxide (O-2(center dot-))-scavenging ability was estimated by an EPR redox probing method. Water suspensions of powdered HST and of its seven constitutive crude drugs were tested. In addition, some of the main water-soluble ingredients of the crude drugs were also tested. HST was found to scavenge both center dot OH and O-2(center dot-). Furthermore, HST was observed to reduce relatively stable nitroxyl radicals. Glycyrrhizae Radix (kanzo), Ginseng Radix (ninjin), Zizyphi Fructus (taiso) and glycyrrhizin (an ingredient of kanzo) were all found to be relatively good center dot OH scavengers. Scutellariae Radix (ogon) and Coptidis Rhizoma (oren) demonstrated reducing ability. In addition, acteoside and berberine chloride, which are water-soluble ingredients of ogon and oren, respectively, also demonstrated reducing ability. Oren exhibited oxidative ability at higher concentrations, which may have a function in maintaining catalytic redox action. The antioxidative function of HST probably worked via a balance of scavenging ROS, reducing stable free radicals, and some minor oxidizing activities.

引用

页码：669 / 677

页数：9